{"id":"W2130114112","doi":"10.1128/aem.02002-06","title":"Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P <i> <sub>mxaF</sub> </i> ) of <i>Methylobacterium extorquens</i> by Applying Regulatory Elements of <i>Pseudomonas putida</i> F1","year":2006,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":39,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Operon; Biology; Pseudomonas putida; Molecular biology; Gene; Repressor; Plasmid; Expression vector; Transposable element; Shuttle vector; Reporter gene; Promoter; Gene expression; Genetics; Vector (molecular biology); Recombinant DNA; Escherichia coli; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004055164,0.0002619279,0.0002947731,0.0000345723,0.00007861006,0.000005476272,0.0001466495,0.0002295397,0.000006157308],"category_scores_gemma":[0.000007684789,0.0002097941,0.00009051426,0.00006135021,0.0003649781,0.000004390028,0.0001443073,0.0001354551,0.000002948499],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002179079,"about_ca_system_score_gemma":0.00001135983,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002227514,"about_ca_topic_score_gemma":0.00000226273,"domain_scores_codex":[0.9985683,0.00008387477,0.0004419233,0.0005540886,0.00006688924,0.0002849188],"domain_scores_gemma":[0.9992676,0.00001230015,0.0002132708,0.000458163,0.000007786782,0.00004089867],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008559843,0.0002064647,0.0005070236,0.00003548157,0.00006250262,3.227836e-7,0.00002117467,0.00002249093,0.997396,0.00004047285,0.0003755292,0.001246934],"study_design_scores_gemma":[0.0004992589,0.000148882,0.0005390591,0.00001189707,0.0000379521,0.00002154623,0.00003724593,0.000001128924,0.9934934,0.0000351466,0.004975335,0.0001991183],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982035,0.0006584298,0.0001002246,0.00003617676,0.0001025205,0.0006227645,0.0002010565,0.00001002455,0.00006529714],"genre_scores_gemma":[0.9986454,0.0001497177,0.0002313561,0.0001916166,0.0001133519,0.00006791778,0.0005469284,0.00002779797,0.00002595131],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004599806,"threshold_uncertainty_score":0.8555154,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003445962455906983,"score_gpt":0.1639098370401681,"score_spread":0.1604638745842612,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}