{"id":"W2130114112","doi":"10.1128/aem.02002-06","title":"Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (P <i> <sub>mxaF</sub> </i> ) of <i>Methylobacterium extorquens</i> by Applying Regulatory Elements of <i>Pseudomonas putida</i> F1","year":2006,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":39,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Operon; Biology; Pseudomonas putida; Molecular biology; Gene; Repressor; Plasmid; Expression vector; Transposable element; Shuttle vector; Reporter gene; Promoter; Gene expression; Genetics; Vector (molecular biology); Recombinant DNA; Escherichia coli; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001948544,0.000676329,0.0003270267,0.0002460451,0.0001337182,0.0002393352,0.0003579289,0.0002310068,0.000948832],"category_scores_gemma":[0.0002117236,0.0002007529,0.0003700319,0.0002567077,0.0002402403,0.0002210761,0.0005154266,0.0007394077,0.0004081007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004296711,"about_ca_system_score_gemma":0.0004119788,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001144297,"about_ca_topic_score_gemma":0.001737639,"domain_scores_codex":[0.9997237,0.0000406483,0.00002414666,0.00006119878,0.00008797643,0.00006220366],"domain_scores_gemma":[0.9997786,0.00005269819,0.00005879109,0.00003506531,0.00003109178,0.00004376573],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001863806,0.000008476304,0.00006038946,0.00001511406,8.715609e-7,0.00001125735,0.000006180614,0.000009017507,0.9994718,0.00001948309,0.000008736001,0.0003701327],"study_design_scores_gemma":[0.00000290917,0.00005344542,0.001064428,0.000002356944,0.000003809482,0.00005782941,0.00001132277,0.0001873928,0.9978915,0.00001169367,0.0007107145,0.000002669928],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9822038,0.0007061171,0.0129718,0.0001787639,0.00005088826,0.0001227909,0.001444256,0.0003165456,0.002004847],"genre_scores_gemma":[0.9688097,0.0008284632,0.02023803,0.00004918587,0.00001584349,0.0001193141,0.004055102,0.0001947958,0.005689615],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001144297,"threshold_uncertainty_score":0.003174186,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003445962455906983,"score_gpt":0.1639098370401681,"score_spread":0.1604638745842612,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}