{"id":"W2130314959","doi":"10.1093/nar/30.2.e9","title":"High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells","year":2002,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1053,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Biology; Transfection; Molecular biology; Green fluorescent protein; HEK 293 cells; Cell culture; Expression vector; Plasmid; Reporter gene; Viral vector; Recombinant DNA; Polyethylenimine; Gene expression; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001175903,0.0009286844,0.00179743,0.000806424,0.0005225497,0.001268036,0.000993309,0.0009420908,0.001991598],"category_scores_gemma":[0.0005789139,0.000459365,0.0009772214,0.001468643,0.0003825197,0.0006563704,0.0007532237,0.00186153,0.003840246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005849883,"about_ca_system_score_gemma":0.000471537,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005946644,"about_ca_topic_score_gemma":0.0008602196,"domain_scores_codex":[0.9991267,0.000137578,0.00008430291,0.0001899926,0.0003662923,0.00009514748],"domain_scores_gemma":[0.9997445,0.00005695833,0.00003130136,0.00005733016,0.00007898438,0.0000308546],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000516539,0.00006395918,0.0000614747,0.0002115099,0.00001469847,0.0000853604,0.00004048288,0.0001953556,0.9949567,0.0002169172,0.0006382039,0.003463667],"study_design_scores_gemma":[0.0000279063,0.0001655412,0.0005531601,0.00002357938,0.00003804015,0.0003835752,0.00002773226,0.003787371,0.9729525,0.0002069196,0.02180929,0.00002427382],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3027773,0.009069995,0.6452208,0.0007546147,0.0006519892,0.002700882,0.01681438,0.00792088,0.01408915],"genre_scores_gemma":[0.4231919,0.01076148,0.4859425,0.0003735368,0.0001774932,0.003967671,0.04683008,0.001713326,0.02704192],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001991598,"threshold_uncertainty_score":0.006662548,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04660526851841845,"score_gpt":0.3111994248175253,"score_spread":0.2645941562991068,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}