{"id":"W2130513750","doi":"10.1186/1472-6750-3-9","title":"The Flp double cross system a simple efficient procedure for cloning DNA fragments","year":2003,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Medical Research Council; Medical Research Council Canada","keywords":"Biology; Multiple cloning site; In vitro recombination; Cloning (programming); FLP-FRT recombination; Polymerase; Recombinase; Genetics; Molecular biology; Molecular cloning; DNA; Recombinant DNA; Computational biology; Vector (molecular biology); Gene; Complementary DNA; Recombination; Genetic recombination; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003893274,0.0002680229,0.000230558,0.00007188056,0.0005324592,0.00007729443,0.0005640959,0.001269801,0.000003997433],"category_scores_gemma":[0.0001850679,0.0002019329,0.0001417571,0.000208762,0.0003749021,0.000001634613,0.0002227778,0.0001962337,0.00001556758],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004344993,"about_ca_system_score_gemma":0.0001505116,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006347588,"about_ca_topic_score_gemma":0.00007546441,"domain_scores_codex":[0.9981331,0.00004313161,0.0003700069,0.0006528859,0.0001044792,0.0006963526],"domain_scores_gemma":[0.9988176,0.00002469047,0.0001797068,0.0008008172,0.0001144703,0.00006276403],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002927076,0.00006970246,0.0007991833,0.0001051551,0.00006761085,0.000001433845,0.00000863476,0.0001511778,0.9630709,0.03403996,0.0005702088,0.0008233487],"study_design_scores_gemma":[0.001306875,0.0003711212,0.0001534447,0.00001192557,0.00001757112,0.00006346939,0.0001540479,0.0002604788,0.7865683,0.0001467026,0.2107197,0.0002262583],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9805371,0.001511541,0.01529201,0.0003761219,0.0007240686,0.001032465,0.00003870225,0.000157916,0.0003301388],"genre_scores_gemma":[0.9946291,0.0001120864,0.004282806,0.00007045735,0.0001380083,0.0002845888,0.00005266289,0.00004651729,0.0003837646],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2101495,"threshold_uncertainty_score":0.9793865,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.011256428184094,"score_gpt":0.2568922950860513,"score_spread":0.2456358669019573,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}