{"id":"W2131384283","doi":"10.1002/bit.25483","title":"Engineering a horseradish peroxidase C stable to radical attacks by mutating multiple radical coupling sites","year":2014,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Enzyme-mediated dye degradation","field":"Agricultural and Biological Sciences","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Korea Carbon Capture and Sequestration R and D Center; National Research Foundation of Korea","keywords":"Chemistry; Radical; Horseradish peroxidase; Mutagenesis; Peroxidase; Protein engineering; Oxidative coupling of methane; Phenylalanine; Site-directed mutagenesis; Mutant; Photochemistry; Biochemistry; Enzyme; Amino acid; Catalysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002940095,0.0004604766,0.0002906598,0.0002660828,0.0001425377,0.0003023763,0.0004194487,0.0005494862,0.0005027606],"category_scores_gemma":[0.0003070043,0.0001840624,0.0004669863,0.00023229,0.000251476,0.000203118,0.0002986792,0.0006828438,0.0002113569],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002781598,"about_ca_system_score_gemma":0.0003199925,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005602343,"about_ca_topic_score_gemma":0.0003977214,"domain_scores_codex":[0.9997788,0.00003073788,0.00003003005,0.00005349705,0.00006761563,0.00003934758],"domain_scores_gemma":[0.9997867,0.00003503778,0.00008999876,0.00002684051,0.00003177186,0.0000296235],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001913388,0.00002170832,0.0001100989,0.00001910835,0.000005994812,0.00004771532,0.000006770149,0.00009577613,0.9986583,0.00006863958,0.00001202982,0.0009347419],"study_design_scores_gemma":[0.00001475345,0.0001462478,0.001007457,0.000003781246,0.00002352662,0.000282836,0.000009065272,0.001399999,0.9958748,0.00003155247,0.001198676,0.000007396107],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9819497,0.0003564034,0.01673648,0.0001027644,0.0000392174,0.00008593975,0.0001013179,0.0001201409,0.0005080685],"genre_scores_gemma":[0.9800854,0.0003343978,0.01802538,0.00005086454,0.00001036551,0.00004302575,0.000221995,0.00004596188,0.001182583],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005602343,"threshold_uncertainty_score":0.002018213,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00699322558677991,"score_gpt":0.1847053163656026,"score_spread":0.1777120907788227,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}