{"id":"W2131384283","doi":"10.1002/bit.25483","title":"Engineering a horseradish peroxidase C stable to radical attacks by mutating multiple radical coupling sites","year":2014,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Enzyme-mediated dye degradation","field":"Agricultural and Biological Sciences","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Korea Carbon Capture and Sequestration R and D Center; National Research Foundation of Korea","keywords":"Chemistry; Radical; Horseradish peroxidase; Mutagenesis; Peroxidase; Protein engineering; Oxidative coupling of methane; Phenylalanine; Site-directed mutagenesis; Mutant; Photochemistry; Biochemistry; Enzyme; Amino acid; Catalysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002790093,0.0002251885,0.0002373647,0.00006887698,0.0001705138,0.00006162999,0.0002091383,0.0004127298,0.00003130936],"category_scores_gemma":[0.0004797079,0.0001227538,0.00004223822,0.0004762718,0.00007441939,0.0001118623,0.0001071504,0.0003311304,0.00002108852],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003432715,"about_ca_system_score_gemma":0.000003222199,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005485485,"about_ca_topic_score_gemma":0.00003970164,"domain_scores_codex":[0.9987013,0.00001369624,0.0002445396,0.0004269772,0.0001394594,0.0004740328],"domain_scores_gemma":[0.9993709,0.0002852952,0.00004327092,0.00008329642,0.00002095298,0.0001962425],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00000879122,0.00002540842,0.002189462,0.00001558797,0.00001494827,0.000003736956,0.00002257103,0.000872727,0.9878356,0.0002391504,0.0003328001,0.008439193],"study_design_scores_gemma":[0.0005663397,0.0004238045,0.006426005,0.000111709,0.00003147126,0.00004414655,0.0001662455,0.3648724,0.5655295,0.00001607197,0.06101991,0.0007924588],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9911731,0.0003398381,0.005440479,0.002143736,0.0001051527,0.0001842043,0.00002809642,0.0005777582,0.000007654307],"genre_scores_gemma":[0.9949948,0.00006502573,0.004526582,0.0001099261,0.000151231,0.00002450947,0.0001050184,0.00000477421,0.00001812453],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4223062,"threshold_uncertainty_score":0.5005753,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00699322558677991,"score_gpt":0.1847053163656026,"score_spread":0.1777120907788227,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}