{"id":"W2131802429","doi":"10.1021/bi702138a","title":"Identification of Residues in DmsD for Twin-Arginine Leader Peptide Binding, Defined through Random and Bioinformatics-Directed Mutagenesis","year":2008,"lang":"en","type":"article","venue":"Biochemistry","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University; University of Calgary","funders":"University of Calgary","keywords":"Translocase; Biochemistry; Peptide sequence; Signal peptide; Mutagenesis; Biology; Amino acid; Peptide; Twin-arginine translocation pathway; Consensus sequence; Molecular biology; Mutant; Gene; Chromosomal translocation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003160112,0.0005561196,0.0004468032,0.0003184067,0.0002659966,0.0003321201,0.0003314776,0.0003135898,0.001070267],"category_scores_gemma":[0.0004400866,0.0002077527,0.0005665625,0.000327728,0.0002641731,0.0001651788,0.0003206264,0.0005273545,0.000659208],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003926245,"about_ca_system_score_gemma":0.0003722186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006760467,"about_ca_topic_score_gemma":0.001293672,"domain_scores_codex":[0.9997236,0.00006756382,0.00003687766,0.00006342871,0.00008174864,0.00002685597],"domain_scores_gemma":[0.9996724,0.00008195685,0.0001075089,0.00003467135,0.00005207115,0.00005136751],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004103105,0.00009650544,0.001891343,0.0001098479,0.00003675191,0.0004445921,0.00005032419,0.0009997616,0.9921873,0.0002888419,0.0001007258,0.003383677],"study_design_scores_gemma":[0.00005531509,0.0004893726,0.01470519,0.00003023782,0.0001451192,0.001668278,0.0000996826,0.009482268,0.96877,0.0001431435,0.004383773,0.00002756069],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9775403,0.0005544557,0.01922359,0.0001052774,0.00005084263,0.000156463,0.001043222,0.0001517978,0.001174205],"genre_scores_gemma":[0.9800884,0.000281008,0.01764046,0.0000624126,0.000008548298,0.00005604402,0.00119601,0.00004899252,0.0006181666],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001070267,"threshold_uncertainty_score":0.003580391,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01434243443998734,"score_gpt":0.2424566205327799,"score_spread":0.2281141860927925,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}