{"id":"W2132425142","doi":"10.1093/protein/gzm066","title":"Expression and enzymatic characterization of the soluble recombinant plasmepsin I from Plasmodium falciparum","year":2007,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Malaria Research and Control","field":"Medicine","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan; University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Recombinant DNA; Plasmodium falciparum; Escherichia coli; Biochemistry; Chemistry; Enzyme; Size-exclusion chromatography; Hemoglobin; Affinity chromatography; Malaria vaccine; Fusion protein; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000321605,0.00008114075,0.0001308741,0.00007330465,0.0000588883,0.00001585566,0.0000280633,0.00006961003,0.00001093674],"category_scores_gemma":[0.0001044189,0.00005805696,0.00001854293,0.0001178635,0.00001436406,0.00006343315,0.00001667694,0.0001359287,6.059423e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000235941,"about_ca_system_score_gemma":0.00002511593,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002835077,"about_ca_topic_score_gemma":0.000001630116,"domain_scores_codex":[0.9993963,0.00003234629,0.0001521518,0.0001229682,0.0001383034,0.0001579951],"domain_scores_gemma":[0.9997314,0.00004976802,0.00004802007,0.00006452853,0.00003863552,0.00006763753],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002970748,0.00002986156,0.0002647795,0.00008087123,0.00001474196,7.827233e-7,0.00008649872,0.00004072585,0.996956,0.0001269657,0.000006781985,0.00209492],"study_design_scores_gemma":[0.0009955765,0.0002148068,0.02913354,0.0002335355,0.00001861828,0.00001079331,0.000007730703,0.1070282,0.8620678,0.0001114866,0.0001121958,0.00006572965],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6793787,0.00007111124,0.3195636,0.0002057994,0.00006486958,0.000676448,6.111495e-7,0.00002888628,0.000009946997],"genre_scores_gemma":[0.9954175,0.00002142196,0.004224892,0.000009757524,0.00004100266,0.00004331063,0.000004310212,0.00001133964,0.0002264726],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3160387,"threshold_uncertainty_score":0.2367494,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01175312455663224,"score_gpt":0.2153840572354003,"score_spread":0.2036309326787681,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}