{"id":"W2132425142","doi":"10.1093/protein/gzm066","title":"Expression and enzymatic characterization of the soluble recombinant plasmepsin I from Plasmodium falciparum","year":2007,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Malaria Research and Control","field":"Medicine","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan; University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Recombinant DNA; Plasmodium falciparum; Escherichia coli; Biochemistry; Chemistry; Enzyme; Size-exclusion chromatography; Hemoglobin; Affinity chromatography; Malaria vaccine; Fusion protein; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001273326,0.0003493993,0.0002250444,0.0001247618,0.0000893415,0.0002123386,0.0001843546,0.0001713665,0.0004061249],"category_scores_gemma":[0.0002151998,0.0001340615,0.0002080336,0.0002894772,0.0001268769,0.0001626557,0.0001695976,0.0004143465,0.0003727352],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002439482,"about_ca_system_score_gemma":0.0001627333,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009001584,"about_ca_topic_score_gemma":0.0006367717,"domain_scores_codex":[0.9999119,0.00001277548,0.0000120101,0.00001677388,0.00002818063,0.00001833014],"domain_scores_gemma":[0.9999285,0.00001160419,0.00002337095,0.000007027699,0.00001530847,0.00001424701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002947142,0.000008742422,0.00009717463,0.00004486015,0.000002699818,0.0000940488,0.00002000182,0.00008023879,0.9986519,0.00005382731,0.00005381673,0.0008631487],"study_design_scores_gemma":[0.00002673114,0.0003039605,0.007858954,0.00001653969,0.00002458519,0.001188839,0.00007995285,0.00191238,0.9794815,0.00009513534,0.008998226,0.00001315762],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9849823,0.002315529,0.008559917,0.0002516411,0.00003879106,0.0001020802,0.002122407,0.00006427863,0.001563206],"genre_scores_gemma":[0.962935,0.003671425,0.01705635,0.0001278096,0.00003901108,0.00008567394,0.01082516,0.00005025385,0.005209373],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009001584,"threshold_uncertainty_score":0.001789808,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01175312455663224,"score_gpt":0.2153840572354003,"score_spread":0.2036309326787681,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}