{"id":"W2133777156","doi":"10.1083/jcb.201404107","title":"pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis","year":2014,"lang":"en","type":"article","venue":"The Journal of Cell Biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":248,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Agence Nationale de la Recherche","keywords":"Endocytosis; Endocytic cycle; Exocytosis; Biology; Internalization; Receptor-mediated endocytosis; Cell biology; Transferrin receptor; Green fluorescent protein; Clathrin; Fluorescence; Live cell imaging; Receptor; Biochemistry; Secretion","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004611496,0.0004003467,0.000301079,0.0005564674,0.0004586389,0.0006146187,0.0006206678,0.0007066925,0.0005802482],"category_scores_gemma":[0.0002575181,0.0003410695,0.0003525863,0.0003305483,0.0005503516,0.0004923965,0.0005808095,0.001360284,0.0003648065],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006272511,"about_ca_system_score_gemma":0.0004046944,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009455667,"about_ca_topic_score_gemma":0.0009613622,"domain_scores_codex":[0.999779,0.00003109823,0.00001174983,0.00004714044,0.00009224266,0.00003868072],"domain_scores_gemma":[0.999882,0.00002819578,0.00002129651,0.00001577704,0.00001905326,0.00003361542],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003089063,0.000008836894,0.0001155236,0.00005087771,0.000003460442,0.00003797939,0.00001398514,0.00006902857,0.995164,0.0006270876,0.000154703,0.003723549],"study_design_scores_gemma":[0.000006937904,0.00004351651,0.000609392,0.000006028192,0.000007077927,0.0005921912,0.00001153305,0.001527638,0.9910271,0.000134416,0.006022932,0.00001129778],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5118566,0.01898423,0.4512591,0.001548927,0.0003000883,0.0004485923,0.001073799,0.001892067,0.01263661],"genre_scores_gemma":[0.6708744,0.01090139,0.3071321,0.0004795558,0.00007995265,0.0002735739,0.001186347,0.0002077108,0.008864963],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009455667,"threshold_uncertainty_score":0.004551053,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005606774914956807,"score_gpt":0.2546436547019591,"score_spread":0.2490368797870023,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}