{"id":"W2134990479","doi":"10.1128/jcm.38.11.4108-4113.2000","title":"PCR Detection of <i>Escherichia coli</i> O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA","year":2000,"lang":"en","type":"article","venue":"Journal of Clinical Microbiology","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":155,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Health Sciences Centre; Sunnybrook Health Science Centre","funders":"","keywords":"Escherichia coli; DNA extraction; Feces; Biology; DNA; Microbiology; Polymerase chain reaction; Bacteria; Enterobacteriaceae; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004822961,0.001244201,0.0008171059,0.001128924,0.0002620117,0.0006301041,0.001112692,0.001005183,0.0008808467],"category_scores_gemma":[0.008857371,0.000558183,0.0004595932,0.0008209872,0.0006912121,0.0006402495,0.0004894931,0.0004671138,0.0005669222],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003208573,"about_ca_system_score_gemma":0.0004032193,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006900751,"about_ca_topic_score_gemma":0.001750904,"domain_scores_codex":[0.995376,0.002068504,0.0006418853,0.0006512702,0.001010329,0.000251877],"domain_scores_gemma":[0.9913981,0.005009102,0.001299525,0.0004737069,0.00161781,0.000201671],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006529263,0.0005173176,0.01936835,0.0009450035,0.00006681962,0.0001665372,0.0002373439,0.000272058,0.9581059,0.00006316564,0.0001324775,0.01947209],"study_design_scores_gemma":[0.0001281507,0.004086827,0.02192008,0.0000806555,0.0002412277,0.0012921,0.0002368378,0.001570402,0.9688506,0.00006129502,0.001491088,0.00004056657],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9242631,0.003893865,0.06919876,0.0002003008,0.0001048292,0.000760786,0.0005258189,0.0002215479,0.0008311237],"genre_scores_gemma":[0.7281122,0.00524095,0.2610725,0.0002794331,0.000112065,0.000925726,0.002720407,0.0001534373,0.001383202],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004822961,"threshold_uncertainty_score":0.02550656,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1077680626978318,"score_gpt":0.4787575015024855,"score_spread":0.3709894388046537,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}