{"id":"W2134990479","doi":"10.1128/jcm.38.11.4108-4113.2000","title":"PCR Detection of <i>Escherichia coli</i> O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA","year":2000,"lang":"en","type":"article","venue":"Journal of Clinical Microbiology","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":155,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Health Sciences Centre; Sunnybrook Health Science Centre","funders":"","keywords":"Escherichia coli; DNA extraction; Feces; Biology; DNA; Microbiology; Polymerase chain reaction; Bacteria; Enterobacteriaceae; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.006323615,0.0001600852,0.0007480639,0.00006318594,0.00007080933,0.000007925777,0.0002472,0.0004497469,0.0000686044],"category_scores_gemma":[0.003659979,0.000137393,0.0004924079,0.0001197994,0.0002806086,0.00001391377,0.00006036571,0.0003959646,0.000001322246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003438,"about_ca_system_score_gemma":0.0002767961,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003559093,"about_ca_topic_score_gemma":0.00008291491,"domain_scores_codex":[0.9960071,0.001807669,0.001560058,0.0002652688,0.0001289893,0.0002308652],"domain_scores_gemma":[0.9963413,0.001134536,0.001129741,0.0002176264,0.001093761,0.00008301305],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002500129,0.0002547681,0.0009247335,0.0000191208,0.0003438245,2.731541e-7,0.00002495845,0.00004173487,0.7987537,0.000001611531,0.0002532012,0.1968819],"study_design_scores_gemma":[0.003610526,0.003317741,0.02442607,0.00005166796,0.0003927398,0.00001820506,0.0000619452,0.0003365573,0.9126737,0.0003441515,0.05459755,0.0001692145],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9840103,0.002369853,0.01219684,0.0001694181,0.0006851453,0.0003592969,0.00005061267,0.000003340458,0.0001551888],"genre_scores_gemma":[0.9753555,0.001237391,0.02235436,0.0001432411,0.0007884734,0.00001856743,0.00003301779,0.000019504,0.00004996865],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1967127,"threshold_uncertainty_score":0.5602722,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1077680626978318,"score_gpt":0.4787575015024855,"score_spread":0.3709894388046537,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}