{"id":"W2135916248","doi":"10.1104/pp.109.149021","title":"Hydrophobin Fusions for High-Level Transient Protein Expression and Purification in<i>Nicotiana benthamiana</i>     ","year":2009,"lang":"en","type":"article","venue":"PLANT PHYSIOLOGY","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":195,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Vineland Research and Innovation Centre; Western University","funders":"Agriculture and Agri-Food Canada; Academy of Finland","keywords":"Hydrophobin; Nicotiana benthamiana; Fusion protein; Green fluorescent protein; Trichoderma reesei; Agroinfiltration; Recombinant DNA; Biology; Agrobacterium tumefaciens; Endoplasmic reticulum; Cell biology; Biochemistry; Chemistry; Transgene; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002071941,0.0003640064,0.0002210508,0.0001628218,0.0001679334,0.0003550778,0.0003014564,0.0003300854,0.0008121886],"category_scores_gemma":[0.0001654444,0.0001465601,0.0001845499,0.0001958147,0.0002310866,0.0003562499,0.000320649,0.0006028754,0.0007109074],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005043754,"about_ca_system_score_gemma":0.000201485,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001297325,"about_ca_topic_score_gemma":0.002391443,"domain_scores_codex":[0.9998379,0.0000279129,0.00001319038,0.00003650618,0.00006358477,0.0000209097],"domain_scores_gemma":[0.9999163,0.00001910526,0.00001968698,0.00001359637,0.00001914893,0.00001211507],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00000398252,0.000002786883,0.00001747344,0.00001387097,8.679749e-7,0.000007896323,0.00000560808,0.00001893507,0.9993611,0.00004904395,0.00002187205,0.0004966783],"study_design_scores_gemma":[0.000002595554,0.00002245729,0.0008164922,0.000002939034,0.000004649671,0.00006402503,0.00001378911,0.0007574034,0.9943997,0.00004633605,0.003866074,0.000003493526],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8667057,0.003773492,0.1199672,0.0005241004,0.0001015653,0.0002385202,0.001715438,0.00186628,0.005107746],"genre_scores_gemma":[0.9437464,0.002607899,0.04095848,0.0001422961,0.00001947283,0.0001694447,0.003091763,0.0002925736,0.008971683],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001297325,"threshold_uncertainty_score":0.003659487,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01387882054606248,"score_gpt":0.2286046367491421,"score_spread":0.2147258162030797,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}