{"id":"W2135916248","doi":"10.1104/pp.109.149021","title":"Hydrophobin Fusions for High-Level Transient Protein Expression and Purification in<i>Nicotiana benthamiana</i>     ","year":2009,"lang":"en","type":"article","venue":"PLANT PHYSIOLOGY","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":195,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Vineland Research and Innovation Centre; Western University","funders":"Agriculture and Agri-Food Canada; Academy of Finland","keywords":"Hydrophobin; Nicotiana benthamiana; Fusion protein; Green fluorescent protein; Trichoderma reesei; Agroinfiltration; Recombinant DNA; Biology; Agrobacterium tumefaciens; Endoplasmic reticulum; Cell biology; Biochemistry; Chemistry; Transgene; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000536036,0.0001150168,0.0001331363,0.00003561536,0.00007507347,0.000006029294,0.000107047,0.0001256699,0.000005042679],"category_scores_gemma":[0.000007893109,0.0001012908,0.00003367025,0.00004388942,0.00003506677,0.000003529171,0.00001181903,0.00006184427,0.000002036735],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005623674,"about_ca_system_score_gemma":0.00002381975,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001966842,"about_ca_topic_score_gemma":0.00006878738,"domain_scores_codex":[0.9992194,0.00002638358,0.0001705586,0.0003439014,0.00004044452,0.0001993799],"domain_scores_gemma":[0.9996788,0.00001271139,0.00004727307,0.0001932779,0.0000150649,0.00005286227],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001284261,0.0001189978,0.00001415832,0.000009858719,0.000005187747,3.42625e-7,0.00003064654,0.00003484211,0.9970326,0.0007884227,0.0006002724,0.001236216],"study_design_scores_gemma":[0.001344258,0.0003808322,0.07391488,0.00003485005,0.0000135409,0.00001025527,0.00003166205,0.0001750633,0.9098958,0.001768517,0.01218042,0.0002499167],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9959118,0.0001967931,0.002064091,0.0005737264,0.00003158917,0.0005658581,0.0005889126,0.00001050226,0.00005676008],"genre_scores_gemma":[0.9968878,0.0001900745,0.001241213,0.0002119708,0.00006902283,0.0001474419,0.001172812,0.000007600471,0.00007203541],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08713682,"threshold_uncertainty_score":0.4130518,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01387882054606248,"score_gpt":0.2286046367491421,"score_spread":0.2147258162030797,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}