{"id":"W2136532823","doi":"10.1038/nchembio.108","title":"A domino effect in antifolate drug action in Escherichia coli","year":2008,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":117,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canada Research Chairs; University of Toronto; University of New Brunswick","funders":"National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences","keywords":"Dihydrofolate reductase; Escherichia coli; Antifolate; Biochemistry; Metabolism; Enzyme; Chemistry; Biology; Antimetabolite; Methotrexate; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002470105,0.0003910545,0.0004264704,0.0004001193,0.0003215073,0.0008877338,0.0002863969,0.0005550295,0.001244709],"category_scores_gemma":[0.0004477784,0.0002195925,0.0001771551,0.0002914073,0.000495048,0.0004174331,0.0003472236,0.0004824456,0.0003604333],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006733342,"about_ca_system_score_gemma":0.000317925,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001102807,"about_ca_topic_score_gemma":0.000823778,"domain_scores_codex":[0.9997063,0.00005076111,0.00001866845,0.00004831083,0.0001066253,0.00006924655],"domain_scores_gemma":[0.9997439,0.0001125947,0.00003016433,0.00002799398,0.00003869171,0.00004659538],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002716376,0.00005225594,0.000273074,0.00006535706,0.000005336601,0.0001300057,0.0000273482,0.0001646749,0.9897069,0.002687581,0.0001466712,0.006469323],"study_design_scores_gemma":[0.00002447829,0.000195438,0.0007440338,0.000004917636,0.00001009906,0.0002581349,0.00003409879,0.001425814,0.99477,0.0006195642,0.001903069,0.00001044362],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9837717,0.002184993,0.004364292,0.0006942357,0.00006620969,0.00002785301,0.0001892728,0.0002783874,0.00842301],"genre_scores_gemma":[0.9960054,0.0006254107,0.0009839099,0.0001006663,0.0000131037,0.000008390832,0.00005049752,0.00002406068,0.002188592],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001244709,"threshold_uncertainty_score":0.004885435,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004455789010517804,"score_gpt":0.2415595089810584,"score_spread":0.2371037199705406,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}