{"id":"W2136532823","doi":"10.1038/nchembio.108","title":"A domino effect in antifolate drug action in Escherichia coli","year":2008,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":117,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canada Research Chairs; University of Toronto; University of New Brunswick","funders":"National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences","keywords":"Dihydrofolate reductase; Escherichia coli; Antifolate; Biochemistry; Metabolism; Enzyme; Chemistry; Biology; Antimetabolite; Methotrexate; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001823726,0.000145574,0.0001958199,0.00007901107,0.00001678858,0.000003837683,0.0001055686,0.0006550383,0.00000765261],"category_scores_gemma":[0.0001693649,0.0001251158,0.00005853412,0.0002234599,0.00006614218,0.000003489593,0.00003553911,0.0005023265,0.000004759697],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002616029,"about_ca_system_score_gemma":0.00002782056,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003418124,"about_ca_topic_score_gemma":0.00001591952,"domain_scores_codex":[0.9991068,0.00007195283,0.0001647534,0.000388859,0.00003458879,0.0002330723],"domain_scores_gemma":[0.9997489,0.00001358675,0.00002659347,0.0001570932,0.0000183119,0.00003544491],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001457271,0.00003315009,0.003207652,0.00002188491,0.00000974933,0.000006006132,0.00001583631,0.000008486074,0.9937615,0.00001528972,0.000612225,0.002162491],"study_design_scores_gemma":[0.0005342501,0.00005053964,0.004179446,0.00001425408,0.000003138121,0.00002863645,0.000001656396,0.00003047675,0.9709923,0.00001336598,0.02400957,0.0001424138],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9954712,0.003558404,0.00004146882,0.0001383308,0.0005406922,0.0001372531,0.000005030106,0.00002216434,0.00008539819],"genre_scores_gemma":[0.9981623,0.0007441838,0.0002716826,0.0001157208,0.0004548827,0.00001536742,0.0001415983,0.00001202173,0.0000822122],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02339734,"threshold_uncertainty_score":0.5102074,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004455789010517804,"score_gpt":0.2415595089810584,"score_spread":0.2371037199705406,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}