{"id":"W2136580306","doi":"10.1128/aem.72.1.384-391.2006","title":"Isolation of Poly-3-Hydroxybutyrate Metabolism Genes from Complex Microbial Communities by Phenotypic Complementation of Bacterial Mutants","year":2006,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":74,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; University of Waterloo; Toyota Research Institute, North America; BIOCAP Canada","keywords":"Complementation; Sinorhizobium meliloti; Biology; Cosmid; Transposon mutagenesis; Mutant; Gene; Genetics; Escherichia coli; Plasmid; Protein-fragment complementation assay; Biochemistry; Transposable element","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002728892,0.0006972193,0.0004119402,0.0004328864,0.000190042,0.0004597869,0.0004880334,0.0003820372,0.0005848639],"category_scores_gemma":[0.0004185305,0.0002211803,0.0005094094,0.0003527092,0.0002446602,0.0001917494,0.0005501385,0.0006799498,0.0004603587],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002624174,"about_ca_system_score_gemma":0.0003526744,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009259771,"about_ca_topic_score_gemma":0.001452597,"domain_scores_codex":[0.9997165,0.00005034407,0.00003996583,0.00005470439,0.00009632964,0.00004222848],"domain_scores_gemma":[0.9995404,0.0001200961,0.0001279579,0.00007872253,0.0000666643,0.00006616532],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002381318,0.00001631762,0.0001666604,0.00001558071,0.00000273155,0.00002561056,0.00001221834,0.00003703067,0.9989268,0.00002655058,0.000003404433,0.0007432092],"study_design_scores_gemma":[0.00001471937,0.0001834576,0.005721145,0.000008745324,0.00002376493,0.0006555613,0.00006800821,0.001465335,0.9908793,0.00006917358,0.00090142,0.000009313984],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.976857,0.0002557799,0.0213307,0.0001160045,0.00001967678,0.0001471007,0.0006732307,0.0001089899,0.0004915209],"genre_scores_gemma":[0.9507138,0.0006596958,0.04270872,0.00009283963,0.00001349178,0.0002168043,0.002950743,0.0001515633,0.002492371],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009259771,"threshold_uncertainty_score":0.001956582,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005693207870935457,"score_gpt":0.1853304727102693,"score_spread":0.1796372648393338,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}