{"id":"W2137696890","doi":"10.1128/aem.70.6.3213-3221.2004","title":"Characterization and Heterologous Gene Expression of a Novel Esterase from<i>Lactobacillus casei</i>CL96","year":2004,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":68,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; McGill University; Biotechnology Research Institute","funders":"","keywords":"Biochemistry; Lactobacillus casei; Biology; Pichia pastoris; Molecular biology; Escherichia coli; Alcohol dehydrogenase; Alcohol oxidase; Heterologous expression; Open reading frame; Esterase; Affinity chromatography; Enzyme; Peptide sequence; Recombinant DNA; Gene; Fermentation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00003002924,0.00012379,0.0001421375,0.00002051832,0.00004449538,0.000005466046,0.00004425934,0.0001484311,0.000008237442],"category_scores_gemma":[0.000002168121,0.0001126485,0.00002332681,0.0000158508,0.0001557634,0.000004344048,0.000110955,0.0000368138,0.00000168129],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009481851,"about_ca_system_score_gemma":0.000004957149,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001205915,"about_ca_topic_score_gemma":0.000004585523,"domain_scores_codex":[0.9993911,0.00001083777,0.000159658,0.0003070272,0.00001890474,0.0001124493],"domain_scores_gemma":[0.9997259,0.000004380871,0.00008513227,0.0001429832,0.000002567293,0.00003902792],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005832012,0.0001010847,0.001298866,0.000005282316,0.00001616299,6.01334e-7,0.00007521884,0.00002862324,0.9979099,0.000006823769,0.0000030714,0.0004960328],"study_design_scores_gemma":[0.001139706,0.0001310173,0.005205499,0.000004873948,0.00001749003,0.00004033366,0.00004866084,0.000003262074,0.9925048,0.000006255666,0.0007831357,0.0001149113],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9931346,0.0002922837,0.006033461,0.00001414706,0.00002931182,0.0001272158,0.000355388,0.000003787226,0.000009805468],"genre_scores_gemma":[0.9920442,0.0003710673,0.0006807629,0.0002575658,0.00003893906,0.00001273502,0.006567609,0.0000117728,0.00001536719],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006212221,"threshold_uncertainty_score":0.4593674,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004427163869345822,"score_gpt":0.1736235221904675,"score_spread":0.1691963583211217,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}