{"id":"W2137696890","doi":"10.1128/aem.70.6.3213-3221.2004","title":"Characterization and Heterologous Gene Expression of a Novel Esterase from<i>Lactobacillus casei</i>CL96","year":2004,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":68,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; McGill University; Biotechnology Research Institute","funders":"","keywords":"Biochemistry; Lactobacillus casei; Biology; Pichia pastoris; Molecular biology; Escherichia coli; Alcohol dehydrogenase; Alcohol oxidase; Heterologous expression; Open reading frame; Esterase; Affinity chromatography; Enzyme; Peptide sequence; Recombinant DNA; Gene; Fermentation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001343452,0.0005030083,0.0003936778,0.0002407413,0.0001440772,0.000562701,0.0003730653,0.0003248831,0.0005874131],"category_scores_gemma":[0.0002180748,0.0001470326,0.0004619837,0.0005903083,0.0001487256,0.0002511226,0.000290629,0.0005461645,0.000511168],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003176329,"about_ca_system_score_gemma":0.0001954903,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008715811,"about_ca_topic_score_gemma":0.0005750394,"domain_scores_codex":[0.9998538,0.00001474947,0.00002058705,0.00003193104,0.00004993615,0.00002889217],"domain_scores_gemma":[0.9998925,0.00001406601,0.00003483169,0.0000164391,0.00001911745,0.00002312082],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003273249,0.00001204712,0.0001179085,0.00002837292,0.000002123578,0.00006506375,0.00001136983,0.00004667579,0.998952,0.00002276281,0.00001533661,0.0006935737],"study_design_scores_gemma":[0.00001669388,0.0001364364,0.00401235,0.00001433959,0.00002147502,0.000927149,0.00007550086,0.000893645,0.9892784,0.00004330958,0.004570525,0.00001013618],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9919153,0.0008743204,0.004115799,0.0001698571,0.00002973297,0.00003724882,0.001423816,0.000079841,0.001354157],"genre_scores_gemma":[0.9746521,0.001196484,0.01233006,0.00008879174,0.00002976412,0.00006983161,0.007771903,0.00008891156,0.003772258],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008715811,"threshold_uncertainty_score":0.002304614,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004427163869345822,"score_gpt":0.1736235221904675,"score_spread":0.1691963583211217,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}