{"id":"W2138254488","doi":"10.1110/ps.9.5.991","title":"Penicillopepsin‐JT2, a recombinant enzyme from <i>Penicillium janthinellum</i> and the contribution of a hydrogen bond in subsite S<sub>3</sub> to k<sub><i>cat</i></sub>","year":2000,"lang":"en","type":"article","venue":"Protein Science","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Biochemistry; Peptide sequence; Aspergillus awamori; Stereochemistry; Chemistry; Amino acid; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008656427,0.0003856551,0.0001522098,0.00009967564,0.00006424739,0.0002164716,0.0001241597,0.000159504,0.0006778483],"category_scores_gemma":[0.0001574469,0.00008684339,0.0001916717,0.0001767697,0.0001177498,0.0001205222,0.00009104004,0.0003782093,0.0002745209],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002090736,"about_ca_system_score_gemma":0.0001435943,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005946516,"about_ca_topic_score_gemma":0.0005732567,"domain_scores_codex":[0.9999311,0.00001218122,0.000007790861,0.00001658652,0.00001981265,0.00001259922],"domain_scores_gemma":[0.9998944,0.00002297404,0.00002975079,0.00001118144,0.0000106389,0.00003104963],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003278827,0.000005852077,0.0001260915,0.00001268034,0.000002211296,0.00002911028,0.000004216105,0.00003078728,0.9993992,0.00001252145,0.000006676962,0.0003378311],"study_design_scores_gemma":[0.00001427316,0.00009106766,0.005607355,0.000004522634,0.00001208691,0.0003453189,0.00001985894,0.0005752191,0.9921097,0.00002181355,0.001195885,0.000002957452],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965044,0.0005360034,0.002076003,0.00003715637,0.00001613824,0.00001511333,0.000288689,0.00002880626,0.0004975937],"genre_scores_gemma":[0.991977,0.0003584687,0.003515583,0.00002381267,0.00000822961,0.0000118614,0.002063445,0.00002622253,0.002015299],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006778483,"threshold_uncertainty_score":0.002267659,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004647861526148139,"score_gpt":0.2081085793264739,"score_spread":0.2034607178003258,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}