{"id":"W2138586548","doi":"10.1093/protein/gzs045","title":"Directed evolution of a  -glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars","year":2012,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Glycoside hydrolase; Directed evolution; Glycosyl; Glycan; Chemistry; Mutant; Biochemistry; Enzyme; Mutagenesis; Glycoside; Stereochemistry; Saturated mutagenesis; Monosaccharide; Glycosyltransferase; Gene; Glycoprotein","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002274592,0.000598353,0.0003106736,0.0003345974,0.000117098,0.0003510903,0.0003865879,0.0003661407,0.0005062659],"category_scores_gemma":[0.0002222514,0.0001769602,0.0003500297,0.000534035,0.0001843923,0.0001304008,0.0003286043,0.0007460297,0.0005188005],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004173054,"about_ca_system_score_gemma":0.0003208435,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001585271,"about_ca_topic_score_gemma":0.001416998,"domain_scores_codex":[0.9998304,0.00002361696,0.0000238354,0.00003591711,0.00004981901,0.00003643342],"domain_scores_gemma":[0.9998205,0.0000346967,0.00004826316,0.00002425295,0.00002560268,0.00004663087],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003476342,0.00002721172,0.0001595735,0.00001602718,0.000006390511,0.00006486008,0.000009392475,0.0001549356,0.9983324,0.00005572821,0.00002260869,0.001116063],"study_design_scores_gemma":[0.00002256092,0.0001506156,0.002914659,0.000006616735,0.000020479,0.0003839996,0.00002251552,0.002885314,0.9912549,0.00005467185,0.00227479,0.000008784723],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9818321,0.0003579766,0.0149086,0.000116503,0.00004782548,0.0001022408,0.0007196133,0.0002821167,0.001632903],"genre_scores_gemma":[0.9639724,0.0004851876,0.02968579,0.00007697046,0.000007520418,0.00006071388,0.001782235,0.0001234876,0.003805655],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001585271,"threshold_uncertainty_score":0.003152013,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01355421591649439,"score_gpt":0.2178763167891046,"score_spread":0.2043221008726102,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}