{"id":"W2138586548","doi":"10.1093/protein/gzs045","title":"Directed evolution of a  -glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars","year":2012,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Glycoside hydrolase; Directed evolution; Glycosyl; Glycan; Chemistry; Mutant; Biochemistry; Enzyme; Mutagenesis; Glycoside; Stereochemistry; Saturated mutagenesis; Monosaccharide; Glycosyltransferase; Gene; Glycoprotein","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001818236,0.0002454905,0.0002736842,0.00007846364,0.00006002077,0.00003187005,0.00008563937,0.0001902247,0.0001276813],"category_scores_gemma":[0.000302147,0.0002696821,0.00006467001,0.0002679346,0.00001382378,0.0002355283,0.0000309336,0.0001892052,0.000006937122],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000229587,"about_ca_system_score_gemma":0.00003258466,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001824725,"about_ca_topic_score_gemma":0.000002268968,"domain_scores_codex":[0.9989157,0.00003501839,0.0002243675,0.0003113067,0.0001597284,0.0003538789],"domain_scores_gemma":[0.9993594,0.0001130797,0.00009565581,0.0001477493,0.00007741866,0.0002067181],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003851037,0.0001223416,0.0000585649,0.000232318,0.00005120581,0.000001056912,0.0001694912,0.0008208203,0.9958414,0.00001126991,0.000009876257,0.002296597],"study_design_scores_gemma":[0.0001878987,0.00002690498,0.0004833696,0.0001310291,0.00004082224,0.000006034369,0.00001053435,0.02774364,0.9710075,0.00001054723,0.0000698738,0.000281889],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8769202,0.0002325689,0.1221944,0.00002300446,0.00005886872,0.0002385538,0.00002382704,0.0002359636,0.00007253254],"genre_scores_gemma":[0.9955171,0.00001395776,0.003912375,0.000002281637,0.000200126,0.0001977521,0.00001010008,0.00003255223,0.0001136986],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1185969,"threshold_uncertainty_score":0.9999756,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01355421591649439,"score_gpt":0.2178763167891046,"score_spread":0.2043221008726102,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}