{"id":"W2139772422","doi":"10.1093/protein/13.1.59","title":"Tryptophan fluorescence of calmodulin binding domain peptides interacting with calmodulin containing unnatural methionine analogues","year":2000,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Heritage Foundation for Medical Research; Fondation pour la Recherche Médicale","keywords":"Calmodulin; Chemistry; Caldesmon; Norleucine; Tryptophan; Methionine; Peptide; Biochemistry; Binding site; Quenching (fluorescence); Myosin light-chain kinase; Fluorescence; Amino acid; Myosin; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001982154,0.0002365001,0.0002647248,0.00007315488,0.0001343247,0.0001915639,0.0002931123,0.0003875964,0.0007240691],"category_scores_gemma":[0.0003386569,0.0001039253,0.0001581099,0.000104452,0.0001613951,0.000181875,0.0001562713,0.0003916849,0.0001534725],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002432182,"about_ca_system_score_gemma":0.00006870165,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003315342,"about_ca_topic_score_gemma":0.0003404821,"domain_scores_codex":[0.9998628,0.00004106951,0.000005419995,0.00003009642,0.00003296317,0.00002759592],"domain_scores_gemma":[0.9998127,0.00008700217,0.00003643635,0.00001300873,0.00002208607,0.00002886363],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001369538,0.0000159203,0.00008965054,0.00001998387,0.00000502815,0.00003053329,0.00001379869,0.00006434222,0.9991509,0.00005463122,0.00001069046,0.0004076542],"study_design_scores_gemma":[0.00002678005,0.0002177364,0.001687423,0.00000663889,0.000007218463,0.0001216467,0.00002042992,0.00193547,0.9951421,0.00004304407,0.0007860094,0.00000550775],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9975308,0.0003730353,0.001693273,0.00001530536,0.00000560214,0.000007374796,0.00002997622,0.00000968083,0.0003351328],"genre_scores_gemma":[0.9966286,0.0002450896,0.002232803,0.00002594335,0.000004152523,0.00001304059,0.0001414114,0.00001319894,0.0006957425],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007240691,"threshold_uncertainty_score":0.002422273,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007334021452755806,"score_gpt":0.2125856318640867,"score_spread":0.2052516104113309,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}