{"id":"W2139772422","doi":"10.1093/protein/13.1.59","title":"Tryptophan fluorescence of calmodulin binding domain peptides interacting with calmodulin containing unnatural methionine analogues","year":2000,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Heritage Foundation for Medical Research; Fondation pour la Recherche Médicale","keywords":"Calmodulin; Chemistry; Caldesmon; Norleucine; Tryptophan; Methionine; Peptide; Biochemistry; Binding site; Quenching (fluorescence); Myosin light-chain kinase; Fluorescence; Amino acid; Myosin; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003391552,0.0001737593,0.0001732111,0.0001011467,0.00009788744,0.00002950575,0.00006007573,0.0001090381,0.00001864714],"category_scores_gemma":[0.0000805271,0.0001575908,0.00003844042,0.0002018143,0.00003520378,0.00001856924,0.00001385146,0.0001588824,0.000001017121],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002202309,"about_ca_system_score_gemma":0.00003056133,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003316596,"about_ca_topic_score_gemma":0.000004860966,"domain_scores_codex":[0.9990887,0.00007992599,0.0002205498,0.0002805507,0.0001210241,0.0002092622],"domain_scores_gemma":[0.9996765,0.00002461407,0.00008059038,0.00008081089,0.00007057462,0.00006690888],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003431391,0.00001699905,0.00009185871,0.0000488601,0.0000395903,0.000002867687,0.00009632006,0.02779353,0.9623551,0.00007735932,0.000002794985,0.009131576],"study_design_scores_gemma":[0.0005743106,0.0005774064,0.0003199344,0.0002612833,0.00001364347,0.00005855283,0.00003691776,0.0722965,0.9253156,0.0000255564,0.0002931397,0.0002271415],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7723461,0.0003412765,0.2269179,0.00002334538,0.00001695412,0.0003055123,0.000001448048,0.0000335531,0.00001389774],"genre_scores_gemma":[0.9107679,0.00002638547,0.08893357,0.000006802332,0.00008748011,0.00004284153,0.00002315594,0.00002246446,0.00008945235],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1384217,"threshold_uncertainty_score":0.6426367,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007334021452755806,"score_gpt":0.2125856318640867,"score_spread":0.2052516104113309,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}