{"id":"W2139775161","doi":"10.1074/mcp.o111.013706","title":"A Human Ubiquitin Conjugating Enzyme (E2)-HECT E3 Ligase Structure-function Screen","year":2012,"lang":"en","type":"article","venue":"Molecular & Cellular Proteomics","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":131,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research; York University","funders":"National Cancer Institute; Canadian Institutes of Health Research; National Center for Research Resources; National Institute of General Medical Sciences; Wellcome Trust","keywords":"Ubiquitin ligase; Ubiquitin; DNA ligase; Chemistry; Function (biology); Enzyme; Biochemistry; Ubiquitin-Protein Ligases; Protein structure; Structure function; Cell biology; Computational biology; Biophysics; Biology; Gene; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003503915,0.0006457819,0.0004698707,0.0004981362,0.0003423457,0.0003102281,0.0003780432,0.00041423,0.001754215],"category_scores_gemma":[0.0002181118,0.0002013809,0.0005922695,0.000386202,0.0001387775,0.0001201874,0.0003209591,0.0003689924,0.0007057294],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005039755,"about_ca_system_score_gemma":0.0004045557,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00131425,"about_ca_topic_score_gemma":0.002893393,"domain_scores_codex":[0.9995635,0.00008088937,0.00003579674,0.00009478361,0.0001693779,0.00005578123],"domain_scores_gemma":[0.9998564,0.00003206157,0.00001804826,0.00002825937,0.00003422506,0.0000310624],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002268741,0.0002645804,0.001866154,0.00005461213,0.00007297694,0.0003922098,0.00001341853,0.0005437616,0.9912889,0.0001424001,0.0004202806,0.004713761],"study_design_scores_gemma":[0.0001185856,0.001227441,0.0168262,0.000006482512,0.0001254612,0.002367793,0.00002838041,0.003550742,0.9673303,0.0001063814,0.008297704,0.00001456239],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9776079,0.001486243,0.01057949,0.0001662034,0.00003340671,0.0003983222,0.004939351,0.0004992344,0.004289783],"genre_scores_gemma":[0.9715676,0.0006966167,0.01266652,0.0002406098,0.000008360316,0.0001597555,0.007889716,0.00008040762,0.006690473],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001754215,"threshold_uncertainty_score":0.005868495,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01146849971264052,"score_gpt":0.2350651110502815,"score_spread":0.223596611337641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}