{"id":"W2140386129","doi":"10.1073/pnas.0600220103","title":"Long-range cooperative binding effects in a T cell receptor variable domain","year":2006,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":66,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lawson Health Research Institute; Western University","funders":"National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Canadian Institutes of Health Research; National Institutes of Health","keywords":"Cooperativity; Mutagenesis; Cooperative binding; Allosteric regulation; Biophysics; Plasma protein binding; Protein structure; Protein domain; Protein–protein interaction; Immunoglobulin domain; Energetics; Alanine scanning; Hot spot (computer programming); Surface plasmon resonance; Biology; Chemistry; Receptor; Mutation; Binding site; Biochemistry; Materials science; Nanotechnology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00120092,0.00009528106,0.0002111459,0.0002468116,0.0001404222,0.00001656143,0.0003296827,0.00007771551,0.00005261303],"category_scores_gemma":[0.0002414452,0.00005804228,0.00006029564,0.001209862,0.0005895916,0.0002085505,0.0001028177,0.0002368556,0.000007080941],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005884971,"about_ca_system_score_gemma":0.0000687201,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006888001,"about_ca_topic_score_gemma":3.569477e-7,"domain_scores_codex":[0.9981245,0.00001189168,0.0002836577,0.0002156734,0.001145121,0.0002191331],"domain_scores_gemma":[0.9993312,0.0002330776,0.0001628934,0.000006019925,0.0002265246,0.00004023869],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001085959,0.0001710437,0.1519042,0.000379502,0.000009371308,8.666375e-8,0.0001223218,0.00003933898,0.7946709,0.05032574,0.002211455,0.00005742475],"study_design_scores_gemma":[0.001084765,0.0002590371,0.2994837,0.0006405252,0.00001382141,0.00001921074,0.0001382715,0.0005603886,0.6732844,0.0231914,0.001183742,0.000140784],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9608318,0.0002559989,0.000001399985,0.002751951,0.00001575078,0.0003297465,0.00001368819,0.000006126249,0.03579357],"genre_scores_gemma":[0.9931048,0.00002513955,0.003754579,0.0001835088,0.0001161169,0.00001676645,5.825718e-7,0.000004282077,0.002794272],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1475794,"threshold_uncertainty_score":0.2366896,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02620212048405283,"score_gpt":0.3141595064325019,"score_spread":0.2879573859484491,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}