{"id":"W2140606746","doi":"10.1002/cyto.a.20167","title":"Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations","year":2005,"lang":"en","type":"article","venue":"Cytometry Part A","topic":"Erythrocyte Function and Pathophysiology","field":"Medicine","cited_by":137,"is_retracted":false,"has_abstract":true,"ca_institutions":"Princess Margaret Cancer Centre","funders":"","keywords":"Epitope; Lysis; Flow cytometry; Molecular biology; Intracellular; Extracellular; Whole blood; Red blood cell; Biology; Phosphorylation; Biochemistry; Chemistry; Antibody; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001291053,0.002260695,0.00116872,0.001385089,0.001003142,0.0007174063,0.001917282,0.001082098,0.01674282],"category_scores_gemma":[0.0007135876,0.0005675564,0.0007250234,0.001156799,0.0008311673,0.0006454902,0.0007520528,0.002440369,0.008631487],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005744855,"about_ca_system_score_gemma":0.001006586,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005898634,"about_ca_topic_score_gemma":0.001164515,"domain_scores_codex":[0.9979674,0.000315431,0.0002816095,0.0006894175,0.0004640835,0.0002819819],"domain_scores_gemma":[0.9994128,0.00009353329,0.0001032211,0.0001933355,0.0001436949,0.00005356872],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0003595049,0.0001508992,0.0004061678,0.0003608706,0.00002458541,0.000144184,0.00009918889,0.0001326757,0.9870427,0.0006540527,0.001807081,0.008818111],"study_design_scores_gemma":[0.0001807459,0.000829054,0.007042931,0.0001180661,0.0001362074,0.001286255,0.00006244604,0.003121241,0.9181769,0.0009008819,0.06805985,0.00008551446],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1932292,0.01124072,0.7428316,0.001083231,0.001923467,0.009722419,0.007815489,0.008281796,0.02387214],"genre_scores_gemma":[0.1834166,0.007565382,0.7405434,0.001041164,0.0004418859,0.02497321,0.01802201,0.001294572,0.02270171],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01674282,"threshold_uncertainty_score":0.05601031,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01518353002089544,"score_gpt":0.2653098972345044,"score_spread":0.2501263672136089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}