{"id":"W2140606746","doi":"10.1002/cyto.a.20167","title":"Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations","year":2005,"lang":"en","type":"article","venue":"Cytometry Part A","topic":"Erythrocyte Function and Pathophysiology","field":"Medicine","cited_by":137,"is_retracted":false,"has_abstract":true,"ca_institutions":"Princess Margaret Cancer Centre","funders":"","keywords":"Epitope; Lysis; Flow cytometry; Molecular biology; Intracellular; Extracellular; Whole blood; Red blood cell; Biology; Phosphorylation; Biochemistry; Chemistry; Antibody; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002815635,0.0002100261,0.0004774341,0.0006309938,0.00009109069,0.00001562563,0.00005836677,0.0002101044,0.0001708263],"category_scores_gemma":[0.0002000306,0.0001824932,0.00008159452,0.001403764,0.00009773575,0.0001678852,0.00002319275,0.0001695494,0.000007987458],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005351614,"about_ca_system_score_gemma":0.00009585075,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001141078,"about_ca_topic_score_gemma":0.00003678826,"domain_scores_codex":[0.9984995,0.0000740036,0.0005415035,0.0004123017,0.0002150035,0.0002576889],"domain_scores_gemma":[0.9989465,0.0001152397,0.0002529829,0.0003094456,0.0002625053,0.0001133416],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00227037,0.00199898,0.221975,0.001028654,0.0001844786,0.00001326157,0.0006472201,0.002671946,0.7649752,0.00003036425,0.0003471375,0.003857324],"study_design_scores_gemma":[0.02186326,0.00338139,0.1532166,0.0003734151,0.0008703411,0.00007665373,0.0003281989,0.03266367,0.7727031,0.00005372298,0.01395203,0.0005176355],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9805613,0.0001436559,0.002365974,0.0002325378,0.0000449932,0.01637784,0.00002502138,0.0000653012,0.0001833559],"genre_scores_gemma":[0.981947,0.00003117056,0.008546103,0.00009140179,0.000177078,0.008189807,0.0003317574,0.00003759166,0.0006480869],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06875838,"threshold_uncertainty_score":0.7441854,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01518353002089544,"score_gpt":0.2653098972345044,"score_spread":0.2501263672136089,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}