{"id":"W2141954515","doi":"10.1128/jb.182.9.2402-2410.2000","title":"Insertion Mutagenesis and Membrane Topology Model of the <i>Pseudomonas aeruginosa</i> Outer Membrane Protein OprM","year":2000,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Medical Research Council; Cystic Fibrosis Foundation","keywords":"Biology; Pseudomonas aeruginosa; Bacterial outer membrane; Mutagenesis; Membrane protein; Membrane topology; Cell biology; Membrane; Mutation; Microbiology; Topology (electrical circuits); Genetics; Bacteria; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002569754,0.0001827371,0.0003911354,0.00006916419,0.0000601622,0.00001047002,0.0003583065,0.0004773576,0.00008226726],"category_scores_gemma":[0.00004526221,0.0001253769,0.0001566914,0.00007711345,0.0003733817,0.000007475891,0.0001328754,0.0001991409,0.000001809138],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001197947,"about_ca_system_score_gemma":0.0001065718,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001572105,"about_ca_topic_score_gemma":0.00002529953,"domain_scores_codex":[0.9987147,0.0001569084,0.0005727865,0.0002269121,0.00009152386,0.0002371523],"domain_scores_gemma":[0.9990886,0.00001089751,0.000378148,0.0003294222,0.0001262131,0.00006668217],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004938163,0.00006273276,0.0003913221,0.00002285142,0.00007846211,0.000004151032,0.00003386277,0.0001553019,0.9921694,0.00006123813,0.00004270546,0.006484125],"study_design_scores_gemma":[0.0008987254,0.0008361282,0.003061844,0.00002257129,0.00005177124,0.0005415712,0.00001573253,0.0001330479,0.9854957,0.0002238605,0.008569758,0.0001492225],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9974497,0.0003504988,0.0000937151,0.001511161,0.0002389676,0.0001748037,0.00002005337,0.000003936096,0.0001571533],"genre_scores_gemma":[0.9966524,0.0009852332,0.00131496,0.0004016862,0.0002060965,0.0000056594,0.000007157527,0.00001790352,0.0004089223],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008527053,"threshold_uncertainty_score":0.5112721,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00820610166799503,"score_gpt":0.2092985746534123,"score_spread":0.2010924729854173,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}