{"id":"W2141954515","doi":"10.1128/jb.182.9.2402-2410.2000","title":"Insertion Mutagenesis and Membrane Topology Model of the <i>Pseudomonas aeruginosa</i> Outer Membrane Protein OprM","year":2000,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Medical Research Council; Cystic Fibrosis Foundation","keywords":"Biology; Pseudomonas aeruginosa; Bacterial outer membrane; Mutagenesis; Membrane protein; Membrane topology; Cell biology; Membrane; Mutation; Microbiology; Topology (electrical circuits); Genetics; Bacteria; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008765307,0.0003004055,0.00015218,0.0001600802,0.0001869128,0.0002627581,0.0003075832,0.000298005,0.0005737626],"category_scores_gemma":[0.0001696807,0.0001210538,0.000252737,0.0001403407,0.0001597175,0.0001622777,0.0001342141,0.0004017708,0.0002652245],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000360399,"about_ca_system_score_gemma":0.0002064333,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005753444,"about_ca_topic_score_gemma":0.0003633915,"domain_scores_codex":[0.9999539,0.0000148172,0.000003673385,0.000008170047,0.00001272342,0.000006716538],"domain_scores_gemma":[0.9999459,0.00001280736,0.0000183366,0.000004884585,0.000006353996,0.00001171464],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001299471,0.00003116593,0.0005465743,0.00004745227,0.000007999449,0.000426344,0.00003457557,0.01298421,0.9808,0.00332204,0.00006119833,0.001608507],"study_design_scores_gemma":[0.0001018776,0.001067708,0.007594082,0.00006729363,0.00008144774,0.001620448,0.0002601867,0.2731434,0.7038338,0.003939517,0.00823354,0.00005669978],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9842285,0.0001548491,0.01397541,0.00009825028,0.00001375552,0.00003659107,0.0001931649,0.00008238638,0.001217177],"genre_scores_gemma":[0.9888396,0.00025878,0.01002403,0.00001819367,0.000003048176,0.00003547083,0.0003363784,0.00001677373,0.0004677075],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005753444,"threshold_uncertainty_score":0.002614915,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00820610166799503,"score_gpt":0.2092985746534123,"score_spread":0.2010924729854173,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}