{"id":"W2142421767","doi":"10.1111/j.1467-7652.2008.00358.x","title":"Cloning and characterization of an acyl‐CoA‐dependent <i>diacylglycerol acyltransferase 1</i> (<i>DGAT1</i>) gene from <i>Tropaeolum majus</i>, and a study of the functional motifs of the DGAT protein using site‐directed mutagenesis to modify enzyme activity and oil content","year":2008,"lang":"en","type":"article","venue":"Plant Biotechnology Journal","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":192,"is_retracted":false,"has_abstract":true,"ca_institutions":"Plant Biotechnology Institute","funders":"","keywords":"Biology; Arabidopsis; Diacylglycerol kinase; Biochemistry; Mutant; Complementary DNA; Acyl-CoA; Open reading frame; Serine; Gene; Molecular biology; Peptide sequence; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008430041,0.0003753302,0.0001506895,0.0001995677,0.0001205876,0.0002477912,0.0001679904,0.000205234,0.0007405501],"category_scores_gemma":[0.0001307959,0.0001199063,0.0002867212,0.0002203585,0.0001383298,0.0001867794,0.0001401038,0.0003694415,0.0006391896],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003729135,"about_ca_system_score_gemma":0.0002633739,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001017683,"about_ca_topic_score_gemma":0.0009327977,"domain_scores_codex":[0.9999461,0.00000474032,0.000005265474,0.00001622355,0.00001452416,0.0000131617],"domain_scores_gemma":[0.999872,0.00001976681,0.00003750417,0.00001215112,0.00002252269,0.00003604529],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002229029,0.000006079465,0.0001697761,0.00002566052,0.000001524692,0.00003308102,0.000009650209,0.00002938541,0.9990121,0.00003940574,0.00001668059,0.0006343055],"study_design_scores_gemma":[0.00001942386,0.000139679,0.01224736,0.00001043578,0.00003021612,0.000804859,0.00004791838,0.000836644,0.9775258,0.00008610777,0.008243061,0.000008605306],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921366,0.001037606,0.004065824,0.0001702592,0.00002289969,0.00003383632,0.001628207,0.0000471706,0.000857443],"genre_scores_gemma":[0.9694696,0.001453221,0.009483393,0.000102076,0.0000222962,0.00004035569,0.01426734,0.00006646013,0.005095216],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001017683,"threshold_uncertainty_score":0.002705693,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01900654335045231,"score_gpt":0.1933870962415586,"score_spread":0.1743805528911063,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}