{"id":"W2142496373","doi":"10.1091/mbc.e02-01-0036","title":"Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses","year":2002,"lang":"en","type":"article","venue":"Molecular Biology of the Cell","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministero dell’Istruzione, dell’Università e della Ricerca; York University; Harvard University","keywords":"Exocytosis; Biology; Synaptophysin; Förster resonance energy transfer; Vesicle; Munc-18; Synaptotagmin 1; Cell biology; Membrane; Biophysics; Energy transfer; Synaptic vesicle; Vesicle fusion; Fluorescence; Biochemistry; Chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000163263,0.0002920718,0.0002846479,0.0002595608,0.0003062606,0.0003588536,0.0005005352,0.0004720823,0.001402644],"category_scores_gemma":[0.0002890939,0.0001448157,0.0001651656,0.000169233,0.0002373357,0.0005173447,0.0002838111,0.0007751003,0.0003649007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000466647,"about_ca_system_score_gemma":0.0001023117,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006367353,"about_ca_topic_score_gemma":0.000627822,"domain_scores_codex":[0.9998881,0.00001164268,0.000003680998,0.00003165984,0.00003889134,0.00002610459],"domain_scores_gemma":[0.9998732,0.00004157102,0.0000211125,0.00001513861,0.00002118576,0.00002782482],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003552192,0.00001133027,0.00009926974,0.00001000969,0.000002689134,0.00006619782,0.00002187378,0.00004090739,0.9990056,0.00007958191,0.00001916525,0.0006078582],"study_design_scores_gemma":[0.00001690578,0.0001292794,0.004514405,0.000006015501,0.00001008891,0.0004759216,0.00007033823,0.003612283,0.9905623,0.0001170207,0.0004766861,0.000008873833],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9906211,0.0005255062,0.007539748,0.00005500684,0.00001245114,0.000009499577,0.0001133958,0.00006901898,0.001054349],"genre_scores_gemma":[0.9878279,0.0005593133,0.008775467,0.00003613632,0.0000147958,0.00003850306,0.0002462114,0.00003201119,0.002469668],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001402644,"threshold_uncertainty_score":0.004692316,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006356310211388537,"score_gpt":0.1782838969459969,"score_spread":0.1719275867346084,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}