{"id":"W2144701572","doi":"10.1128/aem.70.12.7511-7519.2004","title":"Construction and Characterization of a Highly Efficient <i>Francisella</i> Shuttle Plasmid","year":2004,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Bacillus and Francisella bacterial research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":156,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"National Institute of Allergy and Infectious Diseases; Medical College of Wisconsin; Great Lakes Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research","keywords":"Francisella tularensis; Francisella; Tularemia; Plasmid; Biology; Electroporation; Transformation (genetics); Shuttle vector; Microbiology; Escherichia coli; Multiple cloning site; Virology; Genetics; Gene; Expression vector; Vector (molecular biology); Virulence; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004518132,0.0003437436,0.0004191741,0.0003310939,0.0001905915,0.0005017144,0.0005138254,0.0004306895,0.0005082241],"category_scores_gemma":[0.0005626809,0.0001748954,0.0003615303,0.0003702682,0.0002430854,0.0003632827,0.0003477331,0.0008499038,0.0006013013],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003400641,"about_ca_system_score_gemma":0.0002715833,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008777183,"about_ca_topic_score_gemma":0.0007043747,"domain_scores_codex":[0.9997031,0.00004874803,0.00004197876,0.00005180804,0.00009304349,0.00006126992],"domain_scores_gemma":[0.9996972,0.00007828041,0.00008538915,0.0000329923,0.0000589327,0.00004706669],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002166828,0.00003775402,0.0001755766,0.00004363468,0.000004389112,0.00005655337,0.00002414691,0.00008512619,0.9973031,0.0001868267,0.00004926538,0.002012006],"study_design_scores_gemma":[0.00001721522,0.0002616832,0.002245819,0.00001283451,0.00002295913,0.0004110367,0.00003985468,0.001294338,0.98886,0.00005302306,0.006768753,0.00001238625],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9179792,0.0009764676,0.07666363,0.0002963945,0.00009459779,0.0004500069,0.001569247,0.0003923296,0.00157813],"genre_scores_gemma":[0.9142274,0.001229134,0.0670857,0.0001394217,0.00004090604,0.0003011159,0.01261016,0.000216822,0.004149351],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008777183,"threshold_uncertainty_score":0.002467334,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00238270486340915,"score_gpt":0.1621973805101198,"score_spread":0.1598146756467106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}