{"id":"W2144701572","doi":"10.1128/aem.70.12.7511-7519.2004","title":"Construction and Characterization of a Highly Efficient <i>Francisella</i> Shuttle Plasmid","year":2004,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Bacillus and Francisella bacterial research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":156,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"National Institute of Allergy and Infectious Diseases; Medical College of Wisconsin; Great Lakes Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research","keywords":"Francisella tularensis; Francisella; Tularemia; Plasmid; Biology; Electroporation; Transformation (genetics); Shuttle vector; Microbiology; Escherichia coli; Multiple cloning site; Virology; Genetics; Gene; Expression vector; Vector (molecular biology); Virulence; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005091745,0.00009834381,0.0001206614,0.00002260914,0.00005301918,0.000005350512,0.00003988905,0.000122054,0.00001467784],"category_scores_gemma":[0.000001517848,0.00009142778,0.00001876352,0.0000214229,0.0003752706,0.000001391776,0.00008200555,0.00004377575,0.000005329114],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009495,"about_ca_system_score_gemma":0.000009688338,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004859267,"about_ca_topic_score_gemma":0.000001162745,"domain_scores_codex":[0.9994528,0.00001119949,0.0001288943,0.0002391291,0.00002474668,0.0001431821],"domain_scores_gemma":[0.9998076,0.000003749818,0.0000486,0.00009153547,0.000003304001,0.00004518924],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009487389,0.00004228152,0.0003967241,0.00001240513,0.00001405562,3.008979e-7,0.00004588918,0.00001220456,0.9954997,0.0002524281,0.000003242198,0.003625939],"study_design_scores_gemma":[0.001014667,0.0001598623,0.003563731,0.000003945591,0.000007750884,0.00005225903,0.00005135496,0.000003084488,0.9882799,0.00004487322,0.006708697,0.0001099105],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987112,0.0001530823,0.0005898508,0.00003874483,0.00005763946,0.0001468653,0.0001298034,0.000003338992,0.0001694444],"genre_scores_gemma":[0.9985015,0.0005918464,0.000288449,0.00007760843,0.00004248678,0.000008540672,0.000463021,0.000009133691,0.00001738589],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007219791,"threshold_uncertainty_score":0.3728317,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00238270486340915,"score_gpt":0.1621973805101198,"score_spread":0.1598146756467106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}