{"id":"W2145097156","doi":"10.1093/nar/gks396","title":"RF-Cloning.org: an online tool for the design of restriction-free cloning projects","year":2012,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":204,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Cloning (programming); Biology; Genetics; Computational biology; Plasmid; Molecular cloning; Primer (cosmetics); Cloning vector; Restriction site; Restriction enzyme; Computer science; DNA; Programming language; Gene; Complementary DNA; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005975041,0.00342118,0.004329721,0.006316097,0.001669768,0.003391906,0.006487458,0.002129818,0.1083305],"category_scores_gemma":[0.008055709,0.004245958,0.002489868,0.005059338,0.001009636,0.00313842,0.003317116,0.00552738,0.1645233],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009958565,"about_ca_system_score_gemma":0.001903293,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000756811,"about_ca_topic_score_gemma":0.0009189471,"domain_scores_codex":[0.9965134,0.0006932144,0.0006446047,0.0005136824,0.001290558,0.0003445883],"domain_scores_gemma":[0.9959251,0.001832457,0.0005292549,0.0007742477,0.0005405553,0.0003984653],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001927049,0.0005884799,0.001500852,0.005294913,0.0003666049,0.0009522848,0.0005899169,0.002622833,0.1293251,0.01306407,0.5254004,0.3183674],"study_design_scores_gemma":[0.0006261356,0.0002937875,0.001148318,0.000377233,0.0001753558,0.001241218,0.00005180162,0.006216793,0.09036978,0.006697665,0.8925058,0.0002960869],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"methods","genre_gemma":"software","genre_scores_codex":[0.002526036,0.001698479,0.6384096,0.000356455,0.0005046265,0.001338162,0.05717568,0.2898452,0.008145889],"genre_scores_gemma":[0.009976771,0.002559154,0.7010992,0.0004853591,0.0002154616,0.004574933,0.1843434,0.07708611,0.01965955],"genre_candidate":"software","genre_consensus":null,"teacher_disagreement_score":0.1083305,"threshold_uncertainty_score":0.3624015,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1523103070526696,"score_gpt":0.379326161148796,"score_spread":0.2270158540961264,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}