{"id":"W2146299708","doi":"10.1002/bit.21467","title":"Production of lentiviral vectors by large‐scale transient transfection of suspension cultures and affinity chromatography purification","year":2007,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":128,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Biotechnology Research Institute","funders":"","keywords":"Polyethylenimine; Transfection; Viral vector; Bioreactor; Affinity chromatography; Cell culture; Chromatography; Chemistry; Recombinant DNA; Biology; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002624201,0.0000893027,0.0001064552,0.0001354119,0.00004217129,0.000002528331,0.00004733859,0.0002593651,0.000001068124],"category_scores_gemma":[0.00001112061,0.00008342417,0.00003360265,0.0001766198,0.000152994,0.000005032774,0.00001461702,0.00008882976,7.795268e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004081902,"about_ca_system_score_gemma":0.000005667097,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006317178,"about_ca_topic_score_gemma":0.00007608215,"domain_scores_codex":[0.9994215,0.00001579561,0.0001465943,0.0002078782,0.00006384512,0.0001444116],"domain_scores_gemma":[0.9997746,0.000003737749,0.00003675869,0.000127579,0.00003310696,0.00002417507],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002722309,0.00006616415,0.0009482166,0.00006071576,0.00003430303,1.796232e-7,0.00006758315,0.00001087043,0.9956632,0.00002926431,0.00002630092,0.002821006],"study_design_scores_gemma":[0.0002713214,0.0008561696,0.005491516,0.00001457782,0.00001253479,0.000008017782,0.0001237671,0.00003425181,0.9925648,0.000005805872,0.0005392616,0.00007795071],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.992503,0.002679802,0.004410898,0.0001388809,0.00006566905,0.0001614803,0.000009772581,0.00002382268,0.000006671672],"genre_scores_gemma":[0.9981573,0.001522385,0.0002500237,0.00000303861,0.00001974221,0.000003525251,0.0000286269,0.000008006176,0.000007328018],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005654321,"threshold_uncertainty_score":0.3401939,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005879154853923158,"score_gpt":0.237917799584743,"score_spread":0.2320386447308199,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}