{"id":"W2146530830","doi":"10.1128/aem.72.1.506-515.2006","title":"Protease Inhibitors Fail To Prevent Pore Formation by the Activated <i>Bacillus thuringiensis</i> Toxin Cry1Aa in Insect Brush Border Membrane Vesicles","year":2006,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Fonds Québécois de la Recherche sur la Nature et les Technologies","keywords":"Proteases; Biochemistry; Vesicle; EGTA; Protease; Manduca sexta; Brush border; Bacillus thuringiensis; Chemistry; Serine protease; Divalent; Biophysics; Biology; Membrane; Calcium; Enzyme; Bacteria; Insect","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008849815,0.0001807364,0.0001303941,0.00002921147,0.00009771781,0.00001424017,0.0001022788,0.0001567728,0.00001212978],"category_scores_gemma":[0.000002493293,0.0001438433,0.00003336932,0.00005423241,0.0001224551,0.000004673885,0.00011466,0.00009242091,0.000007723533],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003454154,"about_ca_system_score_gemma":0.00001052066,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006667471,"about_ca_topic_score_gemma":0.0001472836,"domain_scores_codex":[0.999115,0.00004198034,0.0002183999,0.0003083098,0.00004026084,0.0002760676],"domain_scores_gemma":[0.9997075,0.000007337673,0.0000632915,0.0001810783,0.000003026441,0.00003777576],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000889842,0.00008486092,0.0002664327,0.00001052313,0.00001103271,0.000001143202,0.0001136049,0.0001100151,0.9954486,0.000007458147,0.001957335,0.001900022],"study_design_scores_gemma":[0.0004840922,0.000114892,0.001091392,0.000005770617,0.000007133417,0.00002332663,0.0002170678,0.000001874369,0.9433944,0.0000248269,0.05445499,0.0001802882],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9983228,0.0005081279,0.00004599449,0.000220674,0.00003021156,0.0005552832,0.00008669318,0.000007099957,0.0002231313],"genre_scores_gemma":[0.9978136,0.0001405656,0.00008686991,0.0006332821,0.00008877288,0.00008652196,0.0009251704,0.00001685245,0.0002083266],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05249765,"threshold_uncertainty_score":0.586576,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002379869873830678,"score_gpt":0.1712022038082192,"score_spread":0.1688223339343885,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}