{"id":"W2146581807","doi":"10.1128/aem.02186-09","title":"Adaptation of the Highly Productive T7 Expression System to <i>Streptomyces lividans</i>","year":2009,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":40,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institut National de la Recherche Scientifique","funders":"","keywords":"T7 RNA polymerase; Biology; Streptomyces; Streptomycetaceae; Actinomycetales; Expression vector; Adaptation (eye); Gene; Gene expression; Genetics; Bacteria; Molecular biology; Bacteriophage; Microbiology; Escherichia coli; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002342267,0.0005580995,0.0004734644,0.0002148777,0.0002388895,0.0004807882,0.0003708309,0.0002967717,0.000608938],"category_scores_gemma":[0.0002720016,0.0001420976,0.0003654017,0.0002833415,0.0002089024,0.0002554286,0.0003773743,0.0009136656,0.0006576612],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002946435,"about_ca_system_score_gemma":0.0003588071,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001001071,"about_ca_topic_score_gemma":0.0009614221,"domain_scores_codex":[0.999756,0.00005357506,0.0000387841,0.00004521013,0.00006008179,0.00004635541],"domain_scores_gemma":[0.9998091,0.0000304592,0.0000372121,0.00003005632,0.00003842381,0.00005487317],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004265699,0.00002999592,0.0001873969,0.00003469478,0.000004300453,0.0000803123,0.00001357905,0.00007512304,0.9974974,0.00009971157,0.00004830076,0.001886594],"study_design_scores_gemma":[0.00001280368,0.0001578708,0.001064222,0.000006227063,0.00001643243,0.0002438802,0.00002590179,0.001390486,0.994244,0.00004336692,0.00278312,0.00001159147],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9406702,0.001134833,0.05349699,0.0002289197,0.000190605,0.0001766409,0.0006456674,0.0006608191,0.002795225],"genre_scores_gemma":[0.9408434,0.0008729891,0.05135681,0.00009712532,0.0000318538,0.00009564015,0.003060324,0.0002382694,0.00340348],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001001071,"threshold_uncertainty_score":0.00213778,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002485648857885867,"score_gpt":0.156636391244453,"score_spread":0.1541507423865671,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}