{"id":"W2147979384","doi":"10.1021/bi000607s","title":"Binding Site Analysis of Cellulose Binding Domain CBD<sub>N1</sub>from Endoglucanse C of<i>Cellulomonas fimi</i>by Site-Directed Mutagenesis","year":2000,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Heteronuclear single quantum coherence spectroscopy; Binding site; Chemistry; Cellulase; Heteronuclear molecule; Alanine; Site-directed mutagenesis; Mutant; Stereochemistry; Biochemistry; Cellulose; Binding domain; Nuclear magnetic resonance spectroscopy; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001394646,0.0003592077,0.0002164462,0.0001052496,0.0001397129,0.0001916801,0.0002972797,0.0002370567,0.0005541291],"category_scores_gemma":[0.0002020535,0.0001162652,0.0002011591,0.000129864,0.0002257994,0.00009284832,0.0001797918,0.0003967021,0.0001769082],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005639739,"about_ca_system_score_gemma":0.0002659728,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00192622,"about_ca_topic_score_gemma":0.001531023,"domain_scores_codex":[0.9998796,0.00002821766,0.00001262806,0.0000234662,0.00004074679,0.00001533612],"domain_scores_gemma":[0.9998626,0.00003820855,0.00004976035,0.00001258783,0.00001995389,0.00001706877],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009725182,0.00002540098,0.0002450569,0.00002799039,0.000004797391,0.00004311287,0.00001351077,0.0002900896,0.9984888,0.00006430523,0.0000122435,0.0006875325],"study_design_scores_gemma":[0.00001200572,0.00007080014,0.00273663,0.000003556432,0.00001223889,0.0001515018,0.00001718248,0.001705716,0.9945127,0.00001686689,0.0007563222,0.00000453543],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965931,0.0001716354,0.002684238,0.00002844431,0.000005620007,0.00002421305,0.0001622246,0.0000166774,0.0003139789],"genre_scores_gemma":[0.9949495,0.0001927524,0.003924373,0.00002636509,0.000002478636,0.00002090119,0.0003887236,0.00001566824,0.0004791441],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00192622,"threshold_uncertainty_score":0.004091978,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004837233981707508,"score_gpt":0.2018517345160368,"score_spread":0.1970145005343293,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}