{"id":"W2147979384","doi":"10.1021/bi000607s","title":"Binding Site Analysis of Cellulose Binding Domain CBD<sub>N1</sub>from Endoglucanse C of<i>Cellulomonas fimi</i>by Site-Directed Mutagenesis","year":2000,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Heteronuclear single quantum coherence spectroscopy; Binding site; Chemistry; Cellulase; Heteronuclear molecule; Alanine; Site-directed mutagenesis; Mutant; Stereochemistry; Biochemistry; Cellulose; Binding domain; Nuclear magnetic resonance spectroscopy; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001665757,0.0002973764,0.0004102356,0.000145373,0.00007747039,0.00002420703,0.0002210786,0.0003337078,0.0005142433],"category_scores_gemma":[0.00005311676,0.0003314518,0.0003112864,0.0008371529,0.0001226916,0.000009700331,0.00006626026,0.0001045108,0.00002686541],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003694274,"about_ca_system_score_gemma":0.00006669981,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006278918,"about_ca_topic_score_gemma":0.00000903045,"domain_scores_codex":[0.9981324,0.00008039953,0.0005530046,0.0006844443,0.0002571487,0.0002925759],"domain_scores_gemma":[0.9987612,0.00002494441,0.0003449753,0.0006098099,0.0001236816,0.000135392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001492576,0.0001688838,0.004880465,0.0000595962,0.0005541741,0.000003484829,0.0001083436,0.00004009567,0.9911911,3.423516e-7,0.001590154,0.00125404],"study_design_scores_gemma":[0.000445117,0.0000414303,0.0007955498,0.0000280013,0.0004295699,0.000003185325,0.00005365271,0.000130017,0.9952255,0.000001351367,0.00251784,0.0003287482],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9967501,0.0009332299,0.0001465043,0.00004300542,0.0000799925,0.0001171518,0.001546466,0.00004587342,0.0003376517],"genre_scores_gemma":[0.985483,0.0006564345,0.0003709188,0.00004575647,0.0001684162,0.00001481716,0.01148074,0.00004023169,0.00173966],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01126711,"threshold_uncertainty_score":0.9999138,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004837233981707508,"score_gpt":0.2018517345160368,"score_spread":0.1970145005343293,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}