{"id":"W2148333082","doi":"10.1016/j.pep.2004.05.011","title":"Molecular cloning, expression, purification, and characterization of fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis—a novel Class II A tetramer","year":2004,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Homotetramer; Aldolase A; Chemistry; Enzyme; Tetramer; Biochemistry; Molecular mass; Enzyme kinetics; Escherichia coli; Recombinant DNA; Fructose-bisphosphate aldolase; Molecular biology; Biology; Active site; Protein subunit; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002336882,0.0003343095,0.0003704042,0.0003061395,0.0003603684,0.0005548848,0.0005061955,0.0003464138,0.0005147907],"category_scores_gemma":[0.0003018792,0.0002916363,0.0003697389,0.00059607,0.0002103403,0.0003991366,0.0002149558,0.000908333,0.0006272604],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006074914,"about_ca_system_score_gemma":0.0004644195,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001884076,"about_ca_topic_score_gemma":0.001601025,"domain_scores_codex":[0.9998223,0.00002424536,0.00002508422,0.00003633631,0.00005879164,0.00003331032],"domain_scores_gemma":[0.9998105,0.00003482285,0.00003581337,0.00002920946,0.00003321239,0.00005650399],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001217049,0.00008132267,0.0002370834,0.0000555433,0.000008146242,0.0000791166,0.0000294065,0.0001385291,0.9963226,0.0003772729,0.0001714076,0.002377784],"study_design_scores_gemma":[0.00003125009,0.0001137164,0.004789559,0.00001315834,0.0000297844,0.0006238348,0.00004636514,0.001887338,0.9745436,0.0002355187,0.01767274,0.00001326962],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9649569,0.00387304,0.02535595,0.001000163,0.0001887169,0.0001193878,0.002904031,0.0001965894,0.001405081],"genre_scores_gemma":[0.9219632,0.004365428,0.04693003,0.0002392303,0.00008278937,0.0001090296,0.01421363,0.0001559768,0.01194082],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001884076,"threshold_uncertainty_score":0.004407644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007299472391503019,"score_gpt":0.2131732504640646,"score_spread":0.2058737780725616,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}