{"id":"W2148333082","doi":"10.1016/j.pep.2004.05.011","title":"Molecular cloning, expression, purification, and characterization of fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis—a novel Class II A tetramer","year":2004,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Homotetramer; Aldolase A; Chemistry; Enzyme; Tetramer; Biochemistry; Molecular mass; Enzyme kinetics; Escherichia coli; Recombinant DNA; Fructose-bisphosphate aldolase; Molecular biology; Biology; Active site; Protein subunit; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002505794,0.0002641944,0.0002771362,0.0001154883,0.0003146608,0.0001114133,0.000161879,0.0002370692,0.0001413897],"category_scores_gemma":[0.0001015048,0.0002278565,0.00004453869,0.0001839839,0.0001448202,0.000623459,0.00009572924,0.0001599953,0.000009647433],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003118044,"about_ca_system_score_gemma":0.0000465938,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001144648,"about_ca_topic_score_gemma":0.00000253459,"domain_scores_codex":[0.9981183,0.00009360468,0.0005675941,0.0006490478,0.0003394181,0.0002319936],"domain_scores_gemma":[0.9986224,0.00002274486,0.0005135913,0.0004821608,0.0002028144,0.0001563314],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001594381,0.0001175894,0.000173311,0.0000713263,0.000006247044,6.9579e-7,0.00106076,0.00001894298,0.9959343,0.001325309,0.00001570396,0.001116392],"study_design_scores_gemma":[0.0009640095,0.00008690088,0.01236959,0.0003222096,0.00002849721,0.000004776318,0.00007892953,0.00007767531,0.9825649,0.001140644,0.002107472,0.0002544288],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9713718,0.0005399637,0.02632597,0.0005173288,0.000226117,0.0007272454,0.0001236045,0.00009517796,0.00007284471],"genre_scores_gemma":[0.9893469,0.0001775307,0.009632084,0.0001171353,0.00009930194,0.0001652888,0.0003394967,0.00003214325,0.00009015657],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01797511,"threshold_uncertainty_score":0.9291719,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007299472391503019,"score_gpt":0.2131732504640646,"score_spread":0.2058737780725616,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}