{"id":"W2148378844","doi":"10.1128/aem.02924-08","title":"Mutations in Domain I Interhelical Loops Affect the Rate of Pore Formation by the <i>Bacillus thuringiensis</i> Cry1Aa Toxin in Insect Midgut Brush Border Membrane Vesicles","year":2009,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Université de Montréal","funders":"","keywords":"Mutant; Bacillus thuringiensis; Manduca sexta; Biochemistry; Midgut; Brush border; Biophysics; Biology; Cysteine; Vesicle; Cell biology; Membrane; Insect; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001839027,0.0001324672,0.0001375051,0.00002714602,0.00006141415,0.000007701922,0.0001254398,0.0001250349,0.00000816753],"category_scores_gemma":[0.000004469166,0.00008931156,0.00003593387,0.00005697365,0.0002556799,0.000003847556,0.00005804206,0.000111386,0.00000220833],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001845857,"about_ca_system_score_gemma":0.000008431176,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001652183,"about_ca_topic_score_gemma":0.0001790332,"domain_scores_codex":[0.9992572,0.00008881394,0.0002276753,0.0002018867,0.00002475238,0.0001996582],"domain_scores_gemma":[0.9997135,0.00002896673,0.0000682519,0.0001688848,0.000002387933,0.00001801528],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009166039,0.00006009178,0.0003959084,0.000006293909,0.00001000236,0.000001040618,0.0004019225,0.000068016,0.9961961,0.00005738604,0.0001974365,0.002514121],"study_design_scores_gemma":[0.001015219,0.0002170654,0.01504196,0.000009318068,0.00001010655,0.00004862311,0.0008380009,0.00001173398,0.9752762,0.0003007848,0.007079979,0.0001510278],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979749,0.0009574954,0.00008409065,0.0004303321,0.00001884987,0.000270351,0.00005455874,0.000002438615,0.0002070308],"genre_scores_gemma":[0.9978882,0.0005183123,0.00005980648,0.001019551,0.00002329235,0.00001974623,0.0004356216,0.000007182912,0.00002826616],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02091994,"threshold_uncertainty_score":0.364202,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00327804268164477,"score_gpt":0.1934918650482808,"score_spread":0.1902138223666361,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}