{"id":"W2148378844","doi":"10.1128/aem.02924-08","title":"Mutations in Domain I Interhelical Loops Affect the Rate of Pore Formation by the <i>Bacillus thuringiensis</i> Cry1Aa Toxin in Insect Midgut Brush Border Membrane Vesicles","year":2009,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"Agriculture and Agri-Food Canada; Université de Montréal","funders":"","keywords":"Mutant; Bacillus thuringiensis; Manduca sexta; Biochemistry; Midgut; Brush border; Biophysics; Biology; Cysteine; Vesicle; Cell biology; Membrane; Insect; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001669002,0.0003281438,0.0001837751,0.0001240555,0.00009318894,0.0002731989,0.0002413462,0.0003189234,0.0008759471],"category_scores_gemma":[0.0002765186,0.0001861979,0.0002942171,0.0001044189,0.0002374718,0.0002107172,0.0001662533,0.0004565217,0.0003883152],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003851993,"about_ca_system_score_gemma":0.0001321624,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001151392,"about_ca_topic_score_gemma":0.0007382344,"domain_scores_codex":[0.9998428,0.00003513954,0.0000314043,0.00003151718,0.00002903571,0.00003008762],"domain_scores_gemma":[0.9997148,0.00007268773,0.0001128712,0.00002411774,0.00002052979,0.00005502577],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001666514,0.00003593666,0.0003521529,0.0000159095,0.000008810277,0.00004562261,0.0000185359,0.0001171475,0.9988149,0.0000393068,0.00001801898,0.0003668701],"study_design_scores_gemma":[0.00002875278,0.0003704159,0.006837729,0.000008360532,0.00002340685,0.0002460875,0.00002914213,0.001558053,0.9903023,0.00002072267,0.0005652905,0.000009744817],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9991845,0.0001160478,0.0003501887,0.00001684672,0.000006562638,0.000008366187,0.00008211357,0.00001995133,0.0002154165],"genre_scores_gemma":[0.9989854,0.0000946737,0.0003681452,0.00001851754,0.000002839416,0.000009498619,0.0001485749,0.00001895494,0.0003534229],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001151392,"threshold_uncertainty_score":0.002930343,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00327804268164477,"score_gpt":0.1934918650482808,"score_spread":0.1902138223666361,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}