{"id":"W2149647571","doi":"10.1016/s0167-4838(02)00304-7","title":"Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum","year":2002,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology","topic":"Proteoglycans and glycosaminoglycans research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"National Heart, Lung, and Blood Institute; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Chondroitin sulfate; Biochemistry; Glucuronic acid; Cleavage (geology); Chondroitin; Stereochemistry; Active site; Binding site; Glycosidic bond; Mutant; Enzyme; Lyase; Oligosaccharide; Glycosaminoglycan; Biology; Polysaccharide","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002172047,0.0003422365,0.0003903249,0.0001457362,0.0003625108,0.0004847895,0.0004798588,0.0005578155,0.00102725],"category_scores_gemma":[0.0003332956,0.0001687425,0.0002830823,0.0001200552,0.0003399764,0.0003769321,0.0002704338,0.0004355679,0.0009694429],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000346418,"about_ca_system_score_gemma":0.0002868951,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001024646,"about_ca_topic_score_gemma":0.0004866277,"domain_scores_codex":[0.999845,0.00003102886,0.00001651448,0.00003698473,0.00003701102,0.00003334922],"domain_scores_gemma":[0.9997573,0.00004320842,0.00006036484,0.00002735126,0.00003731248,0.00007453296],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006771087,0.0000369173,0.0007262708,0.00004384443,0.000009802404,0.00041742,0.00003162658,0.0001487258,0.9960821,0.0002217845,0.00002870662,0.001575698],"study_design_scores_gemma":[0.00004363085,0.0002659465,0.008915553,0.000006154783,0.00002293526,0.0008772226,0.00008347628,0.002125975,0.9861234,0.0002465575,0.001270943,0.00001815093],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9961973,0.0008187682,0.001857846,0.0001125926,0.00002980642,0.000008538166,0.00009510359,0.00003167637,0.0008483485],"genre_scores_gemma":[0.9977647,0.0001667574,0.0008332211,0.00002471825,0.000009761634,0.000003265886,0.0002237797,0.00001043002,0.0009633483],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00102725,"threshold_uncertainty_score":0.003436506,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007222895606449464,"score_gpt":0.230228883024618,"score_spread":0.2230059874181686,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}