{"id":"W2149665909","doi":"10.1002/btpr.272","title":"Asymmetrically simultaneous synthesis of <scp>L</scp>‐homophenylalanine and <i>N</i><sup>6</sup>‐protected‐2‐oxo‐6‐amino‐hexanoic acid by engineered <i>Escherichia coli</i> aspartate aminotransferase","year":2009,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Public Health Agency of Canada","keywords":"Hexanoic acid; Escherichia coli; Chemistry; Lysine; Amino acid; Yield (engineering); Biochemistry; Enzyme; Stereochemistry; Enantioselective synthesis; Catalysis; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001386363,0.0004517322,0.0002732898,0.00008714061,0.00007095326,0.0002125784,0.0002689248,0.0002636583,0.0005128613],"category_scores_gemma":[0.0001605737,0.0001292607,0.0002201403,0.0001590159,0.0001560381,0.0002201701,0.0001953874,0.0003765576,0.0004113825],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002519985,"about_ca_system_score_gemma":0.0002541021,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005598926,"about_ca_topic_score_gemma":0.0008218325,"domain_scores_codex":[0.9998398,0.00002424523,0.00001479875,0.00004086926,0.00004433623,0.00003597631],"domain_scores_gemma":[0.9998697,0.0000144313,0.00004440611,0.00001938842,0.00002237654,0.00002962681],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004463282,0.000008258855,0.00004164356,0.0000163305,0.000002931876,0.00002193848,0.000003931966,0.00005036993,0.9990469,0.00003693267,0.0000127635,0.0007133528],"study_design_scores_gemma":[0.000004661912,0.0000576104,0.0002220824,8.458197e-7,0.000004463016,0.00005559795,0.000003795363,0.0003013831,0.9988697,0.000006282677,0.00047125,0.000002276641],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9833791,0.0006183068,0.01364625,0.00005693792,0.00004140201,0.00004730495,0.0003898983,0.0001199667,0.001700857],"genre_scores_gemma":[0.9842783,0.0004608771,0.01188993,0.0000367741,0.000008232681,0.00003240763,0.0006989551,0.00004409215,0.002550468],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005598926,"threshold_uncertainty_score":0.001828372,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003936898812369029,"score_gpt":0.199530038107197,"score_spread":0.195593139294828,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}