{"id":"W2149806740","doi":"10.1002/bit.20776","title":"Scalable production of adeno‐associated virus type 2 vectors via suspension transfection","year":2006,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"","keywords":"Transfection; HEK 293 cells; Polyethylenimine; Cell culture; Bioreactor; Virus; Adeno-associated virus; Suspension (topology); Gene delivery; Recombinant DNA; Chemistry; Biology; Virology; Gene; Vector (molecular biology); Genetics; Mathematics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000941033,0.0007015825,0.0008194318,0.0006783401,0.0003364684,0.0008690518,0.0007341075,0.0006706946,0.001325661],"category_scores_gemma":[0.0005015538,0.000420399,0.0006078259,0.0003564748,0.0003599502,0.0005265426,0.0006765227,0.00185199,0.001219988],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004445249,"about_ca_system_score_gemma":0.0004720917,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005287852,"about_ca_topic_score_gemma":0.0007272796,"domain_scores_codex":[0.9995029,0.00007135321,0.00006392926,0.0001363639,0.0001725647,0.00005290771],"domain_scores_gemma":[0.9997051,0.000103748,0.00005236383,0.00003982479,0.00005599019,0.00004297496],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001910888,0.00002880687,0.00004304829,0.00003542146,0.000006308368,0.00003203299,0.00002003941,0.0001893231,0.9970216,0.0002389423,0.0001094427,0.002255882],"study_design_scores_gemma":[0.00003192695,0.0002293352,0.0002931823,0.000007706247,0.00002207747,0.0001280662,0.00001246227,0.004126149,0.9902009,0.0002070115,0.004729743,0.00001145714],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3181211,0.003274078,0.6651195,0.0003327891,0.0004298581,0.001371033,0.002376996,0.002591979,0.006382613],"genre_scores_gemma":[0.5090462,0.004398739,0.468054,0.0001740097,0.0001169632,0.001389873,0.006918585,0.0004836364,0.00941799],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001325661,"threshold_uncertainty_score":0.004976749,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007031754120074263,"score_gpt":0.2240719184490793,"score_spread":0.2170401643290051,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}