{"id":"W2150037272","doi":"10.1089/ten.tec.2009.0228","title":"Large-Scale Expansion of Pluripotent Human Embryonic Stem Cells in Stirred-Suspension Bioreactors","year":2009,"lang":"en","type":"article","venue":"Tissue Engineering Part C Methods","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":178,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Heritage Foundation for Medical Research; University of Calgary; California HIV/AIDS Research Program","keywords":"Bioreactor; Embryonic stem cell; Induced pluripotent stem cell; Laboratory flask; Suspension culture; Cell biology; Biology; Stem cell; Flow cytometry; Embryoid body; Cell culture; Regenerative medicine; Mesenchymal stem cell; Chemistry; Molecular biology; Biochemistry; Genetics; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001012332,0.0004687723,0.0004011518,0.0003656571,0.0002678439,0.0004616886,0.0004315914,0.0003375357,0.0009565932],"category_scores_gemma":[0.0004979038,0.0001680556,0.000380717,0.0003257475,0.0002235662,0.0003441402,0.0004327573,0.0006900632,0.001011745],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003564558,"about_ca_system_score_gemma":0.000435005,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006443798,"about_ca_topic_score_gemma":0.001242897,"domain_scores_codex":[0.9995766,0.00007438678,0.00006302531,0.00007039152,0.0001898568,0.00002573709],"domain_scores_gemma":[0.9997682,0.0001015711,0.00002915885,0.000026622,0.0000497876,0.00002461846],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003425727,0.00003189623,0.0001444282,0.00004721426,0.000004282211,0.00005275858,0.00003573224,0.000650594,0.9953901,0.0001987518,0.0001232958,0.003286683],"study_design_scores_gemma":[0.0000381885,0.000268604,0.0009572065,0.00001223024,0.00002536176,0.0001332197,0.0000220945,0.006674901,0.9869524,0.0001936498,0.00470865,0.00001329878],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6205636,0.004517232,0.3593656,0.0005321019,0.0004059527,0.001405521,0.002610474,0.001835655,0.008763775],"genre_scores_gemma":[0.7336503,0.003008833,0.2505392,0.0001846577,0.0001362353,0.0009959136,0.00320739,0.0001871891,0.008090338],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001012332,"threshold_uncertainty_score":0.005353749,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02275238238070831,"score_gpt":0.3285248912736676,"score_spread":0.3057725088929593,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}