{"id":"W2151250250","doi":"10.1128/jb.184.1.76-81.2002","title":"Heterologous Production of <i>Clostridium cellulovorans engB</i> , Using Protease-Deficient <i>Bacillus subtilis</i> , and Preparation of Active Recombinant Cellulosomes","year":2002,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":122,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Cellulosome; Clostridium thermocellum; Biology; Biochemistry; Bacillus subtilis; Recombinant DNA; Molecular biology; Cellulase; Enzyme; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002440961,0.0001307491,0.0003010528,0.0001104868,0.00004309897,0.000006269536,0.00008326517,0.0001525417,0.00002299411],"category_scores_gemma":[0.0001179574,0.0001151539,0.00008892232,0.00009262722,0.0001505878,0.00002444897,0.00003711482,0.0001158946,5.386801e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002022735,"about_ca_system_score_gemma":0.00003922059,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005447345,"about_ca_topic_score_gemma":0.000001862181,"domain_scores_codex":[0.9987891,0.0001789501,0.000580847,0.000215289,0.0001021557,0.0001336414],"domain_scores_gemma":[0.9984713,0.000009479848,0.0009427845,0.0001757343,0.0003530006,0.00004775955],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005637839,0.0002076981,0.0008710913,0.00006023703,0.00005735971,0.000001518214,0.0003058927,0.0001400344,0.9963267,0.000004330513,0.0001098073,0.001351542],"study_design_scores_gemma":[0.0005317018,0.001557956,0.00147798,0.00002864921,0.00005195203,0.0003548588,0.00009498249,0.000108677,0.9935269,0.00001534025,0.002152892,0.0000981035],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.997716,0.0005687681,0.0004041675,0.0002274636,0.0008222029,0.000220015,0.00001110889,0.000003194071,0.00002709261],"genre_scores_gemma":[0.9984912,0.0007524423,0.0003744183,0.00002869591,0.0002194114,0.000003031829,0.00002251321,0.0000126948,0.0000955898],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002799798,"threshold_uncertainty_score":0.4695839,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01622962599656608,"score_gpt":0.2378138014215299,"score_spread":0.2215841754249639,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}