{"id":"W2151250250","doi":"10.1128/jb.184.1.76-81.2002","title":"Heterologous Production of <i>Clostridium cellulovorans engB</i> , Using Protease-Deficient <i>Bacillus subtilis</i> , and Preparation of Active Recombinant Cellulosomes","year":2002,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":122,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Cellulosome; Clostridium thermocellum; Biology; Biochemistry; Bacillus subtilis; Recombinant DNA; Molecular biology; Cellulase; Enzyme; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001997251,0.0004677287,0.0002111726,0.0001591386,0.0001563532,0.0004155322,0.0002567596,0.0002095406,0.0005080822],"category_scores_gemma":[0.0002404209,0.000207296,0.0002060887,0.0003271333,0.0001709616,0.0002205542,0.0002864586,0.0006291455,0.000476913],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005859469,"about_ca_system_score_gemma":0.000341077,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001984468,"about_ca_topic_score_gemma":0.002070776,"domain_scores_codex":[0.9998714,0.00001492875,0.00001549635,0.00002624694,0.00004139237,0.00003053521],"domain_scores_gemma":[0.9998498,0.00002389905,0.00004682609,0.00002636855,0.0000197279,0.00003327317],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003072828,0.00001590398,0.0001197688,0.00003344229,0.00000379148,0.00002841758,0.0000125117,0.00007968897,0.9988292,0.0001530208,0.0000381065,0.0006554748],"study_design_scores_gemma":[0.000009405117,0.00004459028,0.001083136,0.00000579016,0.000007751268,0.00009948995,0.00001457635,0.0003886514,0.9958548,0.00003334509,0.002455299,0.000003199094],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9787254,0.0008453936,0.01609062,0.0001788158,0.00004824992,0.00009432976,0.002418696,0.0001339316,0.001464641],"genre_scores_gemma":[0.9623855,0.0008225334,0.02495601,0.00006406852,0.00001048516,0.00006256391,0.007745535,0.00009027255,0.003863143],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001984468,"threshold_uncertainty_score":0.004251361,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01622962599656608,"score_gpt":0.2378138014215299,"score_spread":0.2215841754249639,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}