{"id":"W2152396858","doi":"10.1351/pac-con-09-01-06","title":"Effect of aberrant disulfide bond formation on protein conformation and molecular property of recombinant therapeutics","year":2010,"lang":"en","type":"article","venue":"Pure and Applied Chemistry","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Recombinant DNA; Fusion protein; Escherichia coli; Disulfide bond; Glutathione; Biochemistry; Glutathione S-transferase; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000483987,0.0002933983,0.0001874654,0.00009577307,0.0001241753,0.0002845076,0.0001423808,0.0002427922,0.00063358],"category_scores_gemma":[0.000650147,0.0001355753,0.0001744246,0.000151052,0.0002317655,0.0002589905,0.0001772226,0.0004316587,0.0001098728],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002159259,"about_ca_system_score_gemma":0.0001593282,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002694771,"about_ca_topic_score_gemma":0.0003186286,"domain_scores_codex":[0.9996506,0.0001303689,0.00004959589,0.00005251506,0.00006790213,0.00004895913],"domain_scores_gemma":[0.9995989,0.0001404671,0.0001132366,0.00005245979,0.00004627697,0.00004873244],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000198397,0.00002389162,0.0004172863,0.00002731122,0.00001440219,0.00009452483,0.00002912671,0.0001378952,0.9980263,0.00008306065,0.00001322058,0.0009345437],"study_design_scores_gemma":[0.000005197397,0.0001671707,0.001513663,0.000001977782,0.00001622976,0.0001523306,0.00001230313,0.0004510515,0.9972287,0.00002773876,0.0004206723,0.000002883829],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998211,0.000342353,0.0009428337,0.0000305261,0.000008956651,0.000008053574,0.00004874909,0.000009510358,0.0003980937],"genre_scores_gemma":[0.998715,0.0002412939,0.000656785,0.00002083132,0.000002827381,0.000004671702,0.00009223698,0.00001019145,0.0002561383],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00063358,"threshold_uncertainty_score":0.002559602,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00370315875955179,"score_gpt":0.2223423688010472,"score_spread":0.2186392100414954,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}