{"id":"W2152794163","doi":"10.1016/s0022-2836(03)00275-4","title":"Probing Catalytically Essential Domain Orientation in Histidine Kinase EnvZ by Targeted Disulfide Crosslinking","year":2003,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research","funders":"National Institutes of Health; National Institute for Health and Care Research; National Institute of General Medical Sciences; Amgen","keywords":"Autophosphorylation; HAMP domain; Dimer; Histidine kinase; Protein subunit; Histidine; Transmembrane domain; Cysteine; Chemistry; Stereochemistry; Helix (gastropod); Crystallography; Biophysics; Biochemistry; Kinase; Biology; Protein kinase A; Binding domain; Binding site; Enzyme; Amino acid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007282965,0.0001677614,0.000249404,0.0001332038,0.00008527515,0.00002162381,0.0001609354,0.000209386,0.0000243701],"category_scores_gemma":[0.000470896,0.0001563634,0.000122048,0.0001939779,0.0001052979,0.000008575503,0.00007494562,0.0001862731,0.000001637774],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005227391,"about_ca_system_score_gemma":0.0001634933,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001013847,"about_ca_topic_score_gemma":0.00000875034,"domain_scores_codex":[0.9984522,0.0002773051,0.0005834983,0.0002576176,0.0001480462,0.0002813165],"domain_scores_gemma":[0.9991649,0.00001527297,0.000351204,0.0001691004,0.0001916813,0.0001078883],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001317715,0.00009211124,0.001468515,0.00001161994,0.00003940278,0.00009388714,0.0000568594,0.0002461489,0.9953299,0.002017211,0.00009891548,0.0004137219],"study_design_scores_gemma":[0.001638319,0.0003076518,0.0001665209,0.00003621417,0.00001992238,0.0001747161,0.00004475686,0.00002797449,0.9889532,0.002839514,0.005594173,0.0001970753],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8413259,0.002289916,0.1558225,0.0001379043,0.0001651387,0.0001404161,0.000006954256,0.000003813333,0.0001074431],"genre_scores_gemma":[0.9928979,0.00004737442,0.006581076,0.0002052655,0.00008966691,0.000006490372,0.0001213311,0.00002067793,0.00003023169],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.151572,"threshold_uncertainty_score":0.6376317,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004719442217148741,"score_gpt":0.2575876870162569,"score_spread":0.2528682447991081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}