{"id":"W2152794163","doi":"10.1016/s0022-2836(03)00275-4","title":"Probing Catalytically Essential Domain Orientation in Histidine Kinase EnvZ by Targeted Disulfide Crosslinking","year":2003,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research","funders":"National Institutes of Health; National Institute for Health and Care Research; National Institute of General Medical Sciences; Amgen","keywords":"Autophosphorylation; HAMP domain; Dimer; Histidine kinase; Protein subunit; Histidine; Transmembrane domain; Cysteine; Chemistry; Stereochemistry; Helix (gastropod); Crystallography; Biophysics; Biochemistry; Kinase; Biology; Protein kinase A; Binding domain; Binding site; Enzyme; Amino acid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002809083,0.0003620021,0.0001609437,0.0001194875,0.0003286585,0.0003913329,0.00032198,0.0003933166,0.001051681],"category_scores_gemma":[0.0002145802,0.000220452,0.0001886071,0.0001255571,0.0003548072,0.0003286441,0.000267002,0.001014067,0.0002727495],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006851543,"about_ca_system_score_gemma":0.0002049651,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006912753,"about_ca_topic_score_gemma":0.001279223,"domain_scores_codex":[0.9999046,0.00002104014,0.000006634891,0.00002462591,0.00002010241,0.00002283335],"domain_scores_gemma":[0.9998134,0.00006098356,0.00005081653,0.00002610169,0.00001667891,0.00003206236],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001331527,0.00002489082,0.0001797529,0.00001538107,0.000004803694,0.00006216178,0.00002003432,0.0001580194,0.9983248,0.0005132854,0.00003612194,0.000527651],"study_design_scores_gemma":[0.0000140075,0.00003109283,0.0008097964,0.000001790151,0.000005455663,0.0001197644,0.0000282029,0.0009879355,0.9973762,0.00009270554,0.0005298906,0.000003138731],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9944246,0.0001829166,0.004232351,0.00009230999,0.00001873248,0.00001091503,0.00009374059,0.00002988518,0.0009146031],"genre_scores_gemma":[0.9970696,0.0001607488,0.001572523,0.00004693331,0.000004855749,0.000008925428,0.0001455553,0.00002245364,0.000968392],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001051681,"threshold_uncertainty_score":0.004971147,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004719442217148741,"score_gpt":0.2575876870162569,"score_spread":0.2528682447991081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}