{"id":"W2152970585","doi":"10.1074/jbc.m203315200","title":"Using 2-Aminopurine Fluorescence to Measure Incorporation of Incorrect Nucleotides by Wild Type and Mutant Bacteriophage T4 DNA Polymerases","year":2002,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":65,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research; Fondation pour la Recherche Médicale; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","keywords":"DNA polymerase; Nucleotide; DNA clamp; Polymerase; DNA; Biology; DNA synthesis; DNA replication; DNA polymerase II; Biochemistry; DNA polymerase I; Base pair; Primase; Molecular biology; Polymerase chain reaction; Reverse transcriptase; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006635914,0.0005837693,0.0005575093,0.0005167489,0.0001757692,0.0005745049,0.0004413693,0.000951634,0.0004771254],"category_scores_gemma":[0.002093369,0.0004141147,0.0004047084,0.0003959983,0.0004757681,0.0005769647,0.0002692937,0.0006033778,0.0002735544],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005480706,"about_ca_system_score_gemma":0.0002127595,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007117775,"about_ca_topic_score_gemma":0.0005160105,"domain_scores_codex":[0.9990867,0.0001889922,0.0001014027,0.0002658107,0.0002657645,0.00009142237],"domain_scores_gemma":[0.9985826,0.0005125486,0.0003440058,0.0001682745,0.0002332377,0.0001593331],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001327376,0.0000221482,0.001125299,0.00003280579,0.00001214112,0.00001762356,0.00001896545,0.0002920395,0.9970281,0.00004495337,0.000007411129,0.001265854],"study_design_scores_gemma":[0.000006751482,0.0001892441,0.002732463,0.000002787027,0.00001088447,0.0001830018,0.00001235461,0.002589855,0.9940211,0.00005317577,0.0001854205,0.00001292857],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9875033,0.000567187,0.01135278,0.0000242622,0.00001235472,0.00001805853,0.000187496,0.0001021419,0.0002323493],"genre_scores_gemma":[0.968084,0.0004894505,0.02998758,0.00003090885,0.000006478926,0.00006345144,0.0005133456,0.0000560231,0.0007686868],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000951634,"threshold_uncertainty_score":0.003976524,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03220844057959008,"score_gpt":0.2382351774721717,"score_spread":0.2060267368925816,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}