{"id":"W2152980897","doi":"10.3791/52148","title":"Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun","year":2014,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Medical Research Council; University of Toronto","keywords":"Transfection; Gene gun; Cell biology; Heterologous; Biology; Gene delivery; Molecular biology; Cell culture; Gene; Nanotechnology; Computational biology; Chemistry; Materials science; Genetics; Plasmid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004658658,0.001068698,0.0004703446,0.0004275456,0.0004937899,0.0004582886,0.001234891,0.000665852,0.001410217],"category_scores_gemma":[0.0001184353,0.0006667026,0.0006496735,0.0003370976,0.0005492692,0.0003553826,0.0005073884,0.001336859,0.001716023],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005792961,"about_ca_system_score_gemma":0.0007903237,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002511811,"about_ca_topic_score_gemma":0.006702682,"domain_scores_codex":[0.9996505,0.00003834616,0.0000352564,0.0001055587,0.0001094904,0.00006078667],"domain_scores_gemma":[0.9998698,0.00002460589,0.00003540256,0.00002659806,0.00001797438,0.00002557052],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001259653,0.000005943602,0.000007338228,0.00003549434,0.000003130511,0.00003365353,0.00001414459,0.0000469123,0.999231,0.0001217141,0.00004896995,0.0004392209],"study_design_scores_gemma":[0.000008327903,0.00005649596,0.0003547286,0.00000631013,0.00001320745,0.0002252342,0.000007601952,0.0004209573,0.9942907,0.00005026638,0.004557865,0.000008311677],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4134959,0.004679197,0.5566638,0.0007244241,0.0005459033,0.001723668,0.005233946,0.005731259,0.01120194],"genre_scores_gemma":[0.3492747,0.008399062,0.6089564,0.0003752812,0.00008154519,0.002165192,0.004690649,0.0009403884,0.02511687],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002511811,"threshold_uncertainty_score":0.004994333,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03060286695481066,"score_gpt":0.396521943337258,"score_spread":0.3659190763824473,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}