{"id":"W2153266303","doi":"10.1074/jbc.m511140200","title":"Scanning the Membrane-bound Conformation of Helix 1 in the Colicin E1 Channel Domain by Site-directed Fluorescence Labeling","year":2005,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"National Institute of General Medical Sciences; National Institutes of Health","keywords":"Colicin; Chemistry; Fluorophore; Helix (gastropod); Fluorescence anisotropy; Fluorescence; Lipid bilayer; Crystallography; Membrane; Biophysics; Fluorescence in the life sciences; Bilayer; Biology; Biochemistry; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006421661,0.0001382018,0.0002098359,0.00001648688,0.0000713495,0.00001834916,0.0003898999,0.0002086669,0.00002842852],"category_scores_gemma":[0.0001566828,0.00006950266,0.0001142042,0.0001284564,0.0001529473,0.000009470797,0.00004815081,0.0002924299,8.437781e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001787903,"about_ca_system_score_gemma":0.00005062989,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008518055,"about_ca_topic_score_gemma":0.000003979294,"domain_scores_codex":[0.9989123,0.00008569402,0.0004926908,0.000122607,0.0001905161,0.0001962355],"domain_scores_gemma":[0.9992286,0.00005455776,0.0003704948,0.0001712057,0.0001281632,0.00004698782],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001240672,0.00005282713,0.00046439,0.00002006836,0.00001500418,0.000002385216,0.0002267433,0.00006048249,0.9978809,0.000001876791,0.0006486689,0.0005025369],"study_design_scores_gemma":[0.0005647764,0.0001115941,0.001220539,0.00003797765,0.00001673866,0.0001387253,0.0004856111,0.00003697503,0.9935842,0.000009967521,0.003696263,0.00009658906],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9983169,0.0006168903,0.00002497185,0.0005716671,0.00005009368,0.00009509114,0.00001999176,0.000004087395,0.0003002942],"genre_scores_gemma":[0.9986174,0.0002637942,0.000222514,0.0003641473,0.0004401052,0.000004026407,0.00004580099,0.000005430467,0.00003681951],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004296706,"threshold_uncertainty_score":0.2834236,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01208216313562132,"score_gpt":0.250574507752256,"score_spread":0.2384923446166347,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}