{"id":"W2153570957","doi":"10.1128/jb.00312-12","title":"Error-Prone PCR Mutagenesis Reveals Functional Domains of a Bacterial Transcriptional Activator, TraJ","year":2012,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Biology; Mutant; Operon; Plasmid; Mutagenesis; Molecular biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002873279,0.0005350816,0.0002703149,0.0002985792,0.0001636642,0.0003148492,0.0003102497,0.0003472096,0.0005866913],"category_scores_gemma":[0.0005255515,0.0002183124,0.0003537039,0.0002195787,0.0002998587,0.0001527531,0.0003071191,0.0006476658,0.0003642456],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003843212,"about_ca_system_score_gemma":0.0002948722,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005580955,"about_ca_topic_score_gemma":0.0009566389,"domain_scores_codex":[0.9996755,0.00008070056,0.00004894942,0.00005632325,0.00008218949,0.00005625496],"domain_scores_gemma":[0.9994051,0.0002277907,0.0001697976,0.00007116479,0.00005174136,0.00007441594],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005743192,0.00002803111,0.0002170896,0.00001999789,0.000007282622,0.0001016273,0.00001701398,0.0001693685,0.9985225,0.00007410098,0.00001511101,0.0007705724],"study_design_scores_gemma":[0.0000108515,0.0001373064,0.003923644,0.000007876472,0.00002975423,0.0007286607,0.00003228248,0.002251747,0.9920216,0.00004236121,0.0008044509,0.000009421403],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9908139,0.0002503785,0.008163755,0.00006414463,0.00002138029,0.00003415954,0.0002084209,0.00005857979,0.0003853004],"genre_scores_gemma":[0.9945589,0.0001652642,0.004185863,0.00003535611,0.000006282562,0.00001569529,0.0003562331,0.00004460324,0.0006317967],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005866913,"threshold_uncertainty_score":0.002788424,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0198723700522586,"score_gpt":0.2416660343310176,"score_spread":0.221793664278759,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}