{"id":"W2154105545","doi":"10.1081/rrs-120014619","title":"THE BRET<sup>2</sup>/ARRESTIN ASSAY IN STABLE RECOMBINANT CELLS: A PLATFORM TO SCREEN FOR COMPOUNDS THAT INTERACT WITH G PROTEIN-COUPLED RECEPTORS (GPCRS)*","year":2002,"lang":"en","type":"article","venue":"Journal of Receptors and Signal Transduction","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":126,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; PerkinElmer Biosignal","funders":"","keywords":"G protein-coupled receptor; HEK 293 cells; Fusion protein; Chemistry; Receptor; Förster resonance energy transfer; Arrestin; Bioluminescence; Aequorin; Green fluorescent protein; Transfection; Cell culture; Luciferase; Biophysics; G protein; Chinese hamster ovary cell; Cell biology; Fluorescence; Recombinant DNA; Biochemistry; Biology; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000907641,0.0002280286,0.0002884079,0.0001117858,0.0002189093,0.0001136519,0.000206156,0.0001405265,0.00006593562],"category_scores_gemma":[0.00003334952,0.0001561399,0.0001208404,0.0001290274,0.00007883361,0.00003409406,0.00001900146,0.0002669809,0.000001615621],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005132942,"about_ca_system_score_gemma":0.00004732333,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005237725,"about_ca_topic_score_gemma":0.0001225353,"domain_scores_codex":[0.9986008,0.00009319813,0.0004502485,0.0002661484,0.0002579665,0.0003315709],"domain_scores_gemma":[0.9992211,0.00004942989,0.000238705,0.0001501072,0.0001830139,0.0001576542],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.004704486,0.000698765,0.004316405,0.0001695275,0.0004252271,0.0000114145,0.007412506,0.01563976,0.8985713,0.00004534964,0.01431737,0.05368796],"study_design_scores_gemma":[0.00637965,0.011458,0.0005487463,0.0005862489,0.0001874688,0.0003438814,0.005878388,0.01348247,0.8052112,0.0004928433,0.1543481,0.001082967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9684793,0.000639669,0.02885877,0.0009038757,0.0004859129,0.0005851783,0.000006576805,0.000005992023,0.00003475969],"genre_scores_gemma":[0.9942495,0.0007737134,0.003802678,0.00006120001,0.0003170926,0.00002224083,0.00001206987,0.0000355857,0.0007259417],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1400307,"threshold_uncertainty_score":0.6367201,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01735172072743562,"score_gpt":0.2187368004941198,"score_spread":0.2013850797666842,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}