{"id":"W2154105545","doi":"10.1081/rrs-120014619","title":"THE BRET<sup>2</sup>/ARRESTIN ASSAY IN STABLE RECOMBINANT CELLS: A PLATFORM TO SCREEN FOR COMPOUNDS THAT INTERACT WITH G PROTEIN-COUPLED RECEPTORS (GPCRS)*","year":2002,"lang":"en","type":"article","venue":"Journal of Receptors and Signal Transduction","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":126,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; PerkinElmer Biosignal","funders":"","keywords":"G protein-coupled receptor; HEK 293 cells; Fusion protein; Chemistry; Receptor; Förster resonance energy transfer; Arrestin; Bioluminescence; Aequorin; Green fluorescent protein; Transfection; Cell culture; Luciferase; Biophysics; G protein; Chinese hamster ovary cell; Cell biology; Fluorescence; Recombinant DNA; Biochemistry; Biology; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002498009,0.003597627,0.003623061,0.001535895,0.0006545759,0.001339585,0.00238748,0.002098807,0.002288133],"category_scores_gemma":[0.0007583628,0.001294811,0.00199595,0.001797677,0.0008769062,0.001120359,0.001173843,0.003530301,0.004845292],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007318492,"about_ca_system_score_gemma":0.0004876665,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004182662,"about_ca_topic_score_gemma":0.000656656,"domain_scores_codex":[0.9974734,0.0006871003,0.0002926828,0.0004653307,0.0008989236,0.0001825374],"domain_scores_gemma":[0.9992662,0.0001952553,0.0001800948,0.0001468661,0.0001526728,0.00005884508],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005850722,0.00003977066,0.00006482783,0.0001129206,0.00001617357,0.00003707475,0.00001598655,0.00008835,0.9975592,0.0001268924,0.0002766899,0.001603633],"study_design_scores_gemma":[0.00002058123,0.0002297418,0.0003853492,0.0000100643,0.00003468847,0.0002622276,0.00000790138,0.002460178,0.9928351,0.00006971371,0.003663346,0.0000210031],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1690728,0.008518958,0.8014346,0.0008193902,0.0004686821,0.001707524,0.005808928,0.007584899,0.004584195],"genre_scores_gemma":[0.2356213,0.009418907,0.7191559,0.000599777,0.0001760003,0.005072972,0.01163205,0.001925785,0.0163973],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003623061,"threshold_uncertainty_score":0.01321095,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01735172072743562,"score_gpt":0.2187368004941198,"score_spread":0.2013850797666842,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}