{"id":"W2154192760","doi":"10.1002/bit.20428","title":"Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis","year":2005,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":115,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Green fluorescent protein; Biochemistry; Valine; Amino acid; Recombinant DNA; Protein biosynthesis; Transfection; Leucine; Asparagine; Chemistry; Gene expression; Casein; Glycine; Molecular biology; Gene; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009617886,0.0007985039,0.001273404,0.0003827461,0.0002848702,0.0008781136,0.0008661056,0.0007278108,0.001985904],"category_scores_gemma":[0.000539313,0.0003667238,0.000601044,0.0008286424,0.0004255035,0.0005674906,0.0006278373,0.001928198,0.002305501],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003920306,"about_ca_system_score_gemma":0.0003148117,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004020718,"about_ca_topic_score_gemma":0.0004387335,"domain_scores_codex":[0.9989735,0.0001993222,0.0001513146,0.0002387229,0.0003153018,0.0001217453],"domain_scores_gemma":[0.9995841,0.0001170275,0.0000716452,0.00007924141,0.0001083277,0.00003966662],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005653898,0.0000291259,0.00004963327,0.00009440285,0.000005030084,0.0000332757,0.00001815138,0.00004240794,0.9985642,0.00004479508,0.00009297171,0.000969581],"study_design_scores_gemma":[0.00001154775,0.0001159063,0.0004961672,0.000008177486,0.00001397273,0.0001179435,0.00001164061,0.0007615883,0.9933455,0.00003250923,0.005077617,0.000007322565],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7325445,0.008179539,0.2341107,0.0008067254,0.0007895809,0.001132776,0.01093413,0.003072269,0.008429738],"genre_scores_gemma":[0.660773,0.008068834,0.2800984,0.00046991,0.0001728599,0.001787739,0.02462352,0.001148832,0.02285693],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001985904,"threshold_uncertainty_score":0.006643534,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00439864267574201,"score_gpt":0.1979976785509346,"score_spread":0.1935990358751926,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}