{"id":"W2154192760","doi":"10.1002/bit.20428","title":"Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis","year":2005,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":115,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Green fluorescent protein; Biochemistry; Valine; Amino acid; Recombinant DNA; Protein biosynthesis; Transfection; Leucine; Asparagine; Chemistry; Gene expression; Casein; Glycine; Molecular biology; Gene; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008916773,0.0001743726,0.0001415101,0.0001491013,0.00008372498,0.00001406085,0.0000816969,0.0004180247,0.00001526964],"category_scores_gemma":[0.00002045971,0.0001612824,0.00005815703,0.0001141553,0.00006015247,0.00001156976,0.00004257557,0.0001467813,0.000003810999],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002158353,"about_ca_system_score_gemma":0.00001305199,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001136315,"about_ca_topic_score_gemma":0.00002364269,"domain_scores_codex":[0.9991161,0.00002608412,0.0001931616,0.0003637465,0.0000571123,0.0002438101],"domain_scores_gemma":[0.9996958,0.000006163603,0.00003613274,0.000189972,0.00001623415,0.0000557311],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006837719,0.00009959856,0.00003907222,0.00003672965,0.00001397747,0.000002721607,0.00001525103,0.0002142189,0.9881704,0.00001688263,0.0001376456,0.01118513],"study_design_scores_gemma":[0.0003509354,0.0002881702,0.0004869426,0.00009156241,0.00001004117,0.00002047195,0.00002905766,0.0004399728,0.9931499,0.00001364941,0.004909639,0.0002096353],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9941487,0.001287168,0.003588086,0.0004058917,0.0001712528,0.0002698842,0.00001850834,0.00008212899,0.0000283616],"genre_scores_gemma":[0.9970009,0.0007433771,0.001881915,0.00003369192,0.0001066308,0.0001371817,0.00002581245,0.00001964301,0.0000508775],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01097549,"threshold_uncertainty_score":0.6576905,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00439864267574201,"score_gpt":0.1979976785509346,"score_spread":0.1935990358751926,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}