{"id":"W2154317105","doi":"10.1128/aem.02214-12","title":"Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration","year":2012,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Clostridium difficile and Clostridium perfringens research","field":"Medicine","cited_by":122,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Institutes of Health Research","keywords":"Biology; Genetics; Plasmid; Gene; Marker gene; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005184446,0.001011386,0.0007434924,0.001111152,0.0006737766,0.0005519018,0.0008372846,0.0007793008,0.001424476],"category_scores_gemma":[0.0003716999,0.0005646637,0.0005312405,0.0009693275,0.00027494,0.0004272949,0.001265497,0.001607803,0.001736692],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003820382,"about_ca_system_score_gemma":0.0006928549,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001059645,"about_ca_topic_score_gemma":0.001267012,"domain_scores_codex":[0.9991595,0.00009212608,0.0001046887,0.0001600103,0.0003326601,0.0001510187],"domain_scores_gemma":[0.9996825,0.00004359698,0.0000820401,0.00007462161,0.00004119193,0.00007604399],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003756298,0.00004448797,0.000112111,0.00006234373,0.00000541806,0.0000578165,0.00003096489,0.00006057788,0.9952142,0.0001987853,0.0001520369,0.004023688],"study_design_scores_gemma":[0.00005006728,0.0003971865,0.002347326,0.00003048311,0.00004887633,0.00108249,0.00004533432,0.001694977,0.9739149,0.0001121703,0.02022086,0.00005532743],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.462282,0.002124683,0.5174509,0.0005960553,0.000471187,0.002173823,0.005361133,0.004578637,0.004961539],"genre_scores_gemma":[0.5967722,0.003097445,0.3611756,0.0002688338,0.00009222295,0.001945183,0.02461808,0.000763574,0.01126688],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001424476,"threshold_uncertainty_score":0.004765272,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02023312740052121,"score_gpt":0.2447289886937704,"score_spread":0.2244958612932492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}