{"id":"W2154568091","doi":"10.1186/s13068-014-0163-1","title":"Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme","year":2014,"lang":"en","type":"article","venue":"Biotechnology for Biofuels","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University; University of Waterloo","funders":"Oak Ridge National Laboratory; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Biology; Shuttle vector; Restriction enzyme; Genetics; Plasmid; Gene; Clostridium acetobutylicum; Mutant; Genome engineering; Intron; Clostridium; Genome; Computational biology; Genome editing; Biochemistry; Recombinant DNA; Vector (molecular biology); Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000382519,0.0007477949,0.000273694,0.0005679923,0.0002515096,0.0003094766,0.000624058,0.0004735197,0.0009498567],"category_scores_gemma":[0.0003375506,0.0002712681,0.0005538569,0.0004842523,0.0003175227,0.0002241033,0.0007876074,0.00143602,0.0006713367],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00045545,"about_ca_system_score_gemma":0.0007343338,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001649395,"about_ca_topic_score_gemma":0.002124255,"domain_scores_codex":[0.9996418,0.00003280195,0.00003562558,0.00006875831,0.0001440125,0.0000770953],"domain_scores_gemma":[0.9996871,0.00004946355,0.00009706241,0.00006364415,0.00004036682,0.00006238234],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002181181,0.00001893529,0.0001427596,0.0000423542,0.000003748134,0.00004089032,0.00001699235,0.000085797,0.9975358,0.0001032686,0.0000493397,0.001938301],"study_design_scores_gemma":[0.00001570885,0.0001581692,0.002045727,0.00001164486,0.0000279332,0.0004459048,0.00002108647,0.001066421,0.9901398,0.00005768701,0.005992811,0.00001706858],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8197173,0.0008985004,0.1705156,0.0005043794,0.0001623233,0.0007061627,0.002431664,0.002124534,0.002939684],"genre_scores_gemma":[0.8047545,0.001271866,0.1837662,0.0001326855,0.00002466705,0.0004037776,0.004676687,0.0004396013,0.004530045],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001649395,"threshold_uncertainty_score":0.003304541,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008683405203917974,"score_gpt":0.1964297964047558,"score_spread":0.1877463912008378,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}