{"id":"W2156363787","doi":"10.1101/pdb.prot085100","title":"Rapid and Efficient Plasmid Construction by Homologous Recombination in Yeast","year":2015,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; Howard Hughes Medical Institute","keywords":"In vitro recombination; Homologous recombination; Plasmid; Cloning (programming); Insert (composites); Molecular cloning; Biology; DNA; Restriction enzyme; Genetics; Cloning vector; Computational biology; Saccharomyces cerevisiae; Recombinant DNA; Ligation; Yeast; Molecular biology; Gene; Computer science; Complementary DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001987938,0.001065684,0.001494879,0.001703944,0.0009225669,0.00135334,0.001782417,0.0009312397,0.002726595],"category_scores_gemma":[0.0008636639,0.0009658861,0.001044722,0.001703186,0.0006512922,0.0008119642,0.001791847,0.002798754,0.006456971],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000602608,"about_ca_system_score_gemma":0.000842544,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007911266,"about_ca_topic_score_gemma":0.001094957,"domain_scores_codex":[0.9981624,0.0002930943,0.0002722929,0.000289118,0.0008077034,0.000175365],"domain_scores_gemma":[0.9996172,0.00006989868,0.00005617984,0.0001076906,0.00007886068,0.00007020137],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000127726,0.0001044775,0.0001316156,0.0005011973,0.00002772729,0.0003166965,0.0001500575,0.0005386763,0.9669124,0.00355373,0.002830981,0.02480475],"study_design_scores_gemma":[0.00009640818,0.000303726,0.0008883991,0.00008756519,0.00005127623,0.001021722,0.000075769,0.002964251,0.8255359,0.001101387,0.1677948,0.00007878564],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.109539,0.01027297,0.8430231,0.000734174,0.0007975954,0.004331208,0.00919134,0.01043137,0.01167924],"genre_scores_gemma":[0.2274758,0.01559136,0.7019842,0.0002753631,0.0001114385,0.002870072,0.02941261,0.002604654,0.01967455],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002726595,"threshold_uncertainty_score":0.01051331,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02137356066978993,"score_gpt":0.2760748877763225,"score_spread":0.2547013271065326,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}