{"id":"W2156363787","doi":"10.1101/pdb.prot085100","title":"Rapid and Efficient Plasmid Construction by Homologous Recombination in Yeast","year":2015,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; Howard Hughes Medical Institute","keywords":"In vitro recombination; Homologous recombination; Plasmid; Cloning (programming); Insert (composites); Molecular cloning; Biology; DNA; Restriction enzyme; Genetics; Cloning vector; Computational biology; Saccharomyces cerevisiae; Recombinant DNA; Ligation; Yeast; Molecular biology; Gene; Computer science; Complementary DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003214423,0.00009464352,0.00009362413,0.00005593955,0.00003614656,0.00003825597,0.0001017098,0.0001053564,0.000005943443],"category_scores_gemma":[0.000100744,0.00009680106,0.00001851299,0.00009045122,0.00008168004,0.000002671773,0.0001071032,0.00009986627,0.000009639213],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003280257,"about_ca_system_score_gemma":0.00009070611,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001676575,"about_ca_topic_score_gemma":0.000004543452,"domain_scores_codex":[0.9991747,0.00006522173,0.0001473427,0.000271,0.0001434382,0.0001983371],"domain_scores_gemma":[0.9995882,0.000005645178,0.00004292988,0.0001687108,0.00008222525,0.000112296],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001513044,0.0001182081,0.006661348,0.0000384218,0.000008119762,0.000003624575,0.00001690072,0.000101263,0.9896882,0.0004778789,0.000614194,0.002120581],"study_design_scores_gemma":[0.001914841,0.0005606896,0.003905921,0.00004917267,0.000002221359,0.000004511589,0.00002327998,0.0009696069,0.9327574,0.00002572958,0.05960089,0.0001857018],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9867008,0.0003573347,0.0002512698,0.0000985367,0.00006616522,0.0120361,0.00001138008,0.00001376398,0.000464673],"genre_scores_gemma":[0.9866241,0.00002510029,0.0007223402,0.0000293779,0.00005288124,0.01241056,0.000004302019,0.00001596337,0.000115419],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0589867,"threshold_uncertainty_score":0.3947433,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02137356066978993,"score_gpt":0.2760748877763225,"score_spread":0.2547013271065326,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}