{"id":"W2156579694","doi":"10.1016/j.bbamem.2003.10.016","title":"Glutamate 87 is important for menaquinol binding in DmsC of the DMSO reductase (DmsABC) from Escherichia coli","year":2003,"lang":"en","type":"article","venue":"Biochimica et Biophysica Acta (BBA) - Biomembranes","topic":"ATP Synthase and ATPases Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research; Swedish Foundation for International Cooperation in Research and Higher Education","keywords":"Chemistry; Mutant; Biochemistry; Periplasmic space; Binding site; Wild type; Escherichia coli; Glutamine; Site-directed mutagenesis; Arginine; Active site; Enzyme; Stereochemistry; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003404941,0.0004148074,0.0004645087,0.000140046,0.0001674395,0.00006563041,0.0006689492,0.0002976239,0.0000530522],"category_scores_gemma":[0.0001888188,0.0003185212,0.0003831038,0.0006106085,0.000381167,0.00001996485,0.0002225153,0.0001878203,0.00001009586],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003376845,"about_ca_system_score_gemma":0.0003076296,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001930264,"about_ca_topic_score_gemma":0.00002978682,"domain_scores_codex":[0.9973522,0.000221363,0.0006465114,0.0007929253,0.0003480215,0.0006390034],"domain_scores_gemma":[0.9983711,0.00008817576,0.0003584222,0.0009263332,0.000106398,0.0001496121],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002917564,0.0003191034,0.00008645085,0.0001007656,0.0001771242,0.000003228183,0.000187795,0.000001578469,0.9973262,0.00009636871,0.00131309,0.00009655957],"study_design_scores_gemma":[0.001168195,0.0002577844,0.0006552853,0.00008658103,0.00005935512,0.000001964824,0.0002533929,0.00008103373,0.9920962,0.0001769887,0.004757652,0.0004055653],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957736,0.0004916053,0.00001073566,0.001076253,0.000229325,0.0008298442,0.001381909,0.00002012931,0.0001865663],"genre_scores_gemma":[0.9974599,0.0006861891,0.0004708923,0.0003688147,0.0001761073,0.0001079455,0.0003833078,0.00007315937,0.0002736405],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005229977,"threshold_uncertainty_score":0.9999267,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0173757242628566,"score_gpt":0.2838964654050877,"score_spread":0.2665207411422311,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}