{"id":"W2157129622","doi":"10.1111/1751-7915.12068","title":"Biochemical studies of the multicopper oxidase (small laccase) from <i> <scp>S</scp> treptomyces coelicolor </i> using bioactive phytochemicals and site‐directed mutagenesis","year":2013,"lang":"en","type":"article","venue":"Microbial Biotechnology","topic":"Enzyme-mediated dye degradation","field":"Agricultural and Biological Sciences","cited_by":55,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Forest Research Institute; University of Toronto","funders":"SLAC National Accelerator Laboratory","keywords":"Multicopper oxidase; Laccase; Myricetin; Streptomyces coelicolor; Caffeic acid; Chemistry; Ferulic acid; Thermostability; Biochemistry; Quercetin; Enzyme; Mutant; Antioxidant; Kaempferol","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000241826,0.0006511518,0.0003435195,0.0002045391,0.0001632487,0.0002869879,0.0001977942,0.0002370148,0.0003879548],"category_scores_gemma":[0.0003219521,0.0001223399,0.0006335751,0.0002846262,0.000216707,0.00022592,0.0001915562,0.0004681092,0.0002110325],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003940898,"about_ca_system_score_gemma":0.0002748047,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002065683,"about_ca_topic_score_gemma":0.001543593,"domain_scores_codex":[0.9997582,0.00004598406,0.0000321218,0.00005381919,0.00006709267,0.00004273774],"domain_scores_gemma":[0.99967,0.00004675841,0.0001112735,0.00002625517,0.00007757814,0.00006803997],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003041295,0.00001609039,0.0001717541,0.00002919685,0.000004834511,0.00002488222,0.000009767043,0.00007517757,0.9989214,0.00001616604,0.000009161638,0.0006912706],"study_design_scores_gemma":[0.000004017066,0.0001169676,0.002930911,0.000002309063,0.00001292859,0.0002355502,0.00001992156,0.0005073174,0.9955748,0.00001371566,0.0005756657,0.000005855224],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9876364,0.0009650005,0.009934312,0.0001265317,0.00002393579,0.00004638228,0.0005950298,0.00008852118,0.0005839206],"genre_scores_gemma":[0.9879981,0.0006429844,0.00890171,0.00003731715,0.000008508935,0.00002603188,0.00152616,0.00003704088,0.0008220688],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002065683,"threshold_uncertainty_score":0.004107296,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02248174448775517,"score_gpt":0.2230994284404006,"score_spread":0.2006176839526455,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}