{"id":"W2157198824","doi":"10.1016/j.jbiotec.2013.11.007","title":"The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody","year":2013,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":116,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Glycosylation; Chemistry; Monoclonal antibody; Antibody; Intracellular; Nucleotide sugar; Biochemistry; Glycan; Chinese hamster ovary cell; Cell culture; Oligosaccharide; Molecular biology; Glycoprotein; Biology; Enzyme; Biosynthesis; Immunology; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006164422,0.0001289069,0.000233098,0.00004042637,0.0001731679,0.00002596344,0.0003324773,0.0002297195,0.00001885818],"category_scores_gemma":[0.0004627104,0.00006230742,0.0001510398,0.0001737132,0.0005263928,0.00001017315,0.0001936771,0.0002318809,0.00000396624],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001485979,"about_ca_system_score_gemma":0.00007319861,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007489048,"about_ca_topic_score_gemma":0.00002111181,"domain_scores_codex":[0.9988519,0.0001767468,0.0004728289,0.000161024,0.0001523228,0.0001851897],"domain_scores_gemma":[0.9985547,0.0001181623,0.0005906536,0.0004198975,0.0002582763,0.00005829143],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003779503,0.00007251142,0.002345019,0.00002036168,0.00006904884,4.944632e-7,0.00004944812,0.0000503951,0.9908693,0.0002647881,0.002848833,0.00303187],"study_design_scores_gemma":[0.0007308118,0.0005678382,0.01311636,0.00002821449,0.0000411967,0.00003007383,0.00007489955,0.0002448018,0.9800076,0.000634649,0.004448685,0.00007490857],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9916061,0.002039711,0.002805205,0.00263781,0.0002418948,0.0005932807,0.00005847806,0.000005069894,0.0000124504],"genre_scores_gemma":[0.9982061,0.001397592,0.0001229134,0.00007294869,0.0001309999,0.00002494749,0.00001434367,0.000007678304,0.00002245438],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01086172,"threshold_uncertainty_score":0.2540823,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003713081107134648,"score_gpt":0.2320773428291744,"score_spread":0.2283642617220397,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}