{"id":"W2159694046","doi":"10.1006/prep.2000.1356","title":"Bacterial Expression of an Immunologically Reactive PCV2 ORF2 Fusion Protein","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Animal Virus Infections Studies","field":"Agricultural and Biological Sciences","cited_by":44,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Polyclonal antibodies; Fusion protein; Maltose-binding protein; Western blot; Molecular biology; Affinity chromatography; Protein A/G; Porcine circovirus; Protein G; Biology; Open reading frame; Immunoprecipitation; Myc-tag; Escherichia coli; Protein purification; Chemistry; Antibody; Recombinant DNA; Virology; Biochemistry; Peptide sequence; Gene; Virus","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002612739,0.0001654936,0.0001909514,0.00002516686,0.0004107053,0.00004310159,0.000167664,0.0001762514,0.0004683596],"category_scores_gemma":[0.0001360132,0.00006717459,0.00004734798,0.0002036948,0.0001140368,0.0003325426,0.0001224052,0.0001418159,0.00001442565],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001907972,"about_ca_system_score_gemma":0.000006560951,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001275747,"about_ca_topic_score_gemma":0.0000239853,"domain_scores_codex":[0.9986809,0.0002347604,0.000305161,0.0003862215,0.0002048257,0.0001881117],"domain_scores_gemma":[0.9993542,0.00004548187,0.0002654754,0.0001148513,0.0001433949,0.00007655228],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004527869,0.0002282199,0.0005531585,0.000007192873,0.000002573492,7.887694e-7,0.0001145258,3.017253e-7,0.9479364,0.0001673788,0.00001940824,0.05051726],"study_design_scores_gemma":[0.0002162647,0.0005886808,0.1036285,0.0001375412,0.000004593186,0.000004763425,0.000323905,0.00001576802,0.8860164,0.0008672301,0.008031509,0.0001648117],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973623,0.0001685888,0.00007037307,0.0002630992,0.00004125818,0.0009133018,0.00001306174,0.0001009131,0.001067135],"genre_scores_gemma":[0.9978877,0.0001076919,0.001230894,0.00001406797,0.0001442602,0.0002791325,0.0000623939,0.000001919861,0.0002719479],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1030754,"threshold_uncertainty_score":0.5128209,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02651608993963354,"score_gpt":0.2402954558945386,"score_spread":0.2137793659549051,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}