{"id":"W2159694046","doi":"10.1006/prep.2000.1356","title":"Bacterial Expression of an Immunologically Reactive PCV2 ORF2 Fusion Protein","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Animal Virus Infections Studies","field":"Agricultural and Biological Sciences","cited_by":44,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Polyclonal antibodies; Fusion protein; Maltose-binding protein; Western blot; Molecular biology; Affinity chromatography; Protein A/G; Porcine circovirus; Protein G; Biology; Open reading frame; Immunoprecipitation; Myc-tag; Escherichia coli; Protein purification; Chemistry; Antibody; Recombinant DNA; Virology; Biochemistry; Peptide sequence; Gene; Virus","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004258956,0.001037047,0.0005669492,0.0005082493,0.000711431,0.000862263,0.0005430855,0.0008678615,0.001459188],"category_scores_gemma":[0.0003904345,0.0003957738,0.0007005689,0.0008924596,0.00053124,0.0005316143,0.0006209186,0.001915184,0.001462806],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001520856,"about_ca_system_score_gemma":0.0006503933,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0032247,"about_ca_topic_score_gemma":0.002751569,"domain_scores_codex":[0.9995797,0.00005648443,0.00003547829,0.00008039005,0.0001243229,0.0001235974],"domain_scores_gemma":[0.9996762,0.00009079508,0.00003889619,0.00005221213,0.00006392145,0.00007804396],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001218897,0.00005301554,0.0001055841,0.00005634236,0.00001091428,0.00006254679,0.00003391933,0.0001005826,0.9985185,0.0003124141,0.0001430633,0.0004811546],"study_design_scores_gemma":[0.000016347,0.00007347303,0.001104928,0.000005391001,0.00001850503,0.0001767463,0.00003258145,0.001327212,0.9932981,0.00005949732,0.003879634,0.000007621905],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.958014,0.00142799,0.02961039,0.00060763,0.0003898136,0.0002481051,0.004479907,0.0004333878,0.004788779],"genre_scores_gemma":[0.9426106,0.0009921482,0.02152758,0.0002534243,0.00008970765,0.0002326229,0.01679544,0.0002721054,0.01722632],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0032247,"threshold_uncertainty_score":0.01103467,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02651608993963354,"score_gpt":0.2402954558945386,"score_spread":0.2137793659549051,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}