{"id":"W2160474225","doi":"10.1128/aem.68.3.1102-1108.2002","title":"Engineering of a <i>Bacillus subtilis</i> Strain with Adjustable Levels of Intracellular Biotin for Secretory Production of Functional Streptavidin","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Biotin; Streptavidin; Bacillus subtilis; Biochemistry; Operon; Intracellular; Biology; Chemistry; Escherichia coli; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006332156,0.0001065145,0.0001707932,0.00002481011,0.00002466772,6.81331e-7,0.00003633908,0.0001394223,0.00003524751],"category_scores_gemma":[0.000003982952,0.00008900227,0.00003505285,0.00002465563,0.0002685502,0.000001667901,0.00003778169,0.00004982592,4.132885e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006544016,"about_ca_system_score_gemma":0.000004650398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000001932657,"about_ca_topic_score_gemma":0.000001475384,"domain_scores_codex":[0.999463,0.000008533638,0.0001767087,0.0002093912,0.00002329286,0.0001191214],"domain_scores_gemma":[0.9997691,0.000009122108,0.0001002459,0.00009640489,0.000006866413,0.0000182528],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007759174,0.00008807068,0.0003741965,0.00008633166,0.0001224038,1.138342e-7,0.00007828478,0.000333772,0.9975879,0.00004958385,0.0001254618,0.001076241],"study_design_scores_gemma":[0.0005978871,0.000307612,0.001275618,0.00001176156,0.0000361919,0.00001691142,0.000333652,0.00002916035,0.9949248,0.000003781556,0.002365271,0.00009731729],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968492,0.001885336,0.00059141,0.00002308559,0.0000564517,0.0002029571,0.0003330448,0.000002726104,0.00005577196],"genre_scores_gemma":[0.9984492,0.0003484922,0.0007796563,0.000009176323,0.00003538756,0.000008111078,0.0001735186,0.00000995224,0.0001864801],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002663113,"threshold_uncertainty_score":0.3629407,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007126187372585022,"score_gpt":0.1495224871075812,"score_spread":0.1423962997349962,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}