{"id":"W2160474225","doi":"10.1128/aem.68.3.1102-1108.2002","title":"Engineering of a <i>Bacillus subtilis</i> Strain with Adjustable Levels of Intracellular Biotin for Secretory Production of Functional Streptavidin","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Biotin; Streptavidin; Bacillus subtilis; Biochemistry; Operon; Intracellular; Biology; Chemistry; Escherichia coli; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002494393,0.0006084499,0.0005286525,0.0003065185,0.0002208815,0.000528118,0.0004829514,0.0004101447,0.0007299762],"category_scores_gemma":[0.0002663732,0.000297525,0.0003904827,0.0004411974,0.0002592848,0.0001928327,0.0005244699,0.0007275313,0.0007456953],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004892344,"about_ca_system_score_gemma":0.0004251512,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002324739,"about_ca_topic_score_gemma":0.002314455,"domain_scores_codex":[0.9996408,0.00005554327,0.00005893567,0.00005530488,0.0001262443,0.00006311837],"domain_scores_gemma":[0.9996938,0.00005185511,0.0001023172,0.00003134978,0.0000420517,0.00007862947],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003227152,0.00003248901,0.00008813063,0.00002676878,0.000002407994,0.00004948293,0.00001559704,0.0001026486,0.9987729,0.00009021774,0.00003477779,0.0007523618],"study_design_scores_gemma":[0.000018369,0.0001582717,0.001283443,0.00001194709,0.00001131142,0.0002500645,0.00004541068,0.001677573,0.9934852,0.00003587388,0.003010339,0.00001228084],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9672744,0.0005576682,0.02488742,0.0003551506,0.00009287347,0.0002133609,0.001499015,0.0004534485,0.004666766],"genre_scores_gemma":[0.9414074,0.0007601732,0.0473851,0.0001095282,0.00001515766,0.0001810655,0.002608909,0.0003362171,0.007196472],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002324739,"threshold_uncertainty_score":0.004622459,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007126187372585022,"score_gpt":0.1495224871075812,"score_spread":0.1423962997349962,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}